酵素および稀酸加水分解を併用したキシランからキシロースの調製法 : Streptomyces属のXylanase系に関する研究 (第3報)

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This paper concerns the preparation of xylose from hardwood xylan, by a combined process of enzymatic hydrolysis by extracellular xylanase from the Streptomyces sp. E-86 and dilute-acid hydrolysis.Optimum temperature for xylan liquefaction by this enxyme was about 55〜60℃, maximal total sugar concentration of the solution obtained was 30%w/v. In the final stage of hydrolysis, the average degree of polymerization of the hydrolyzates decreased to about 1.6.The enzymatic hydrolyzate was treated with Amberlite IR-200 (H^+ form) and HCl was added to 0.15% based on the sugar solution. After hydrolysis for 3 hr at 100℃, the average degree of polymerization of the enzymatic hydrolysis products was lowered to 1.06 and the concentration of the solution obtained was 23% w/v.As a typical experimental production of xylose, 1,400g or xylan were liquefied by incubation with xylanase solution at 60℃ for 8hr and 55℃ for 22hr, and the supernatant was decationized with Amberilite IR-200 (H^+ form) column. Then 310g of 1N HCl solution was added to the decationized hydrolyzate of total weight 7,025g, containing 1,405g of xylose, and the acidified hydrolyzate was heated at 100℃ for 3hr. The resulting hydrolyzate was decolorized with charcoal and concentrated by evaporation, and crude crystalline xylose (1.165 g as xylose) was obtained.
社団法人日本生物工学会の論文
1975-03-25