醗酵生産物の分離からみたイタコン酸醗酵原料の検討 : 醗酵生産物の分離に関する研究(第6報)

概要

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For industrial production of itaconic acid, it would be desirable to employ cheaper raw materials such as high test molasses, blackstrap molasses and sometimes raw sugar, provided that high yield with satisfactory speed of fermentation could be attained.With raw sugar, acid production approaching the normal fermentation rate and yield was realized by the addition of nutrients. With high test molasses, removal of the potassium ion was essential, and with blackstrap molasses, organic acids also had to be removed to obtain about 50% acid yield from the sugar consumed.The optimal pH values of medium ranged from the 2 to 6 for raw sugar, 2.5 to 3.5 for high test, and around 3.5 for blackstrap molasses. Control of pH values around 2.2 in the acid accumulation phase gave high fermentation rate and acid yield.Recovery of itaconic acid crystals from the solution fermented with glucose, raw sugar, cation-exchanged hightest, and cation-exchanged and ether-treated blackstrap molasses was accomplished by a simple evaporation-crystallization procedure, and 81 to 96% of the total acid was recovered in the form of 95 to 98% tan crystals.
社団法人日本生物工学会の論文
1975-05-25