イタコン酸醗酵に関する研究 : (第5報)連続培養に関する基礎的研究 : (その3)単槽定常連続培養におけるイタコン酸集積速度, 菌体増殖速度におよぼす培養温度およびCSL供給速度の効果

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The effects of cultural temperature of Asp. terreus k 26 and the feeding rate of corn steep liquor, on the average specific rates of itaconic acid production and the mycelial growth in a single stage steady state type operation are summarized in Table 1.The effect of cultural temperature on the average specific rate of acid production and mycelial growth will be seen in Figs. 2 and 3. Taking temperature below 36℃, the average specific rates of acid production and the mycelial growth increase with temperature were represented by the following empirical equations.(Vi)/(Cm)=6.82・10^<19>・exp {(-13540)/T} (1) (Vm)/(Cm)=4.75・10^<15>・exp {(-11230)/T} (2) where 297.5<T<308,1.4<Ci<1.5,0.55<(Vn)/(Cm)<0.66 The values of theramal increment were found to be 22,300 cal per mole for the mycelial growth, and 26,900 cal per mole for the acid production, respectively.The effect of the change in cultural temperature on average specific rate of the acid production is given in Fig. 4.Owing to the absence of corn steep liquor in the feeding medium, the acid productivity of the mycelium grown at the optimum temperature decreased with the lapse of time. When the logarithm of the average specific rates of acid production are plotted against the reciprocal of the absolute temperature, a atraight line relation found to give the following emprical equation. (Refer to Fig. 5) (Vi)/(Cm)=1.93・10^<10>・exp {(-6,720)/T} (3) The value of Q_<10> was calculated as 2.07 between 26° and 36℃.The relation between the acid concentration in the fermenting broth and the specific rates of acid production and mycelial growth was found as is shown in Figs. 6 and 7. Relations given by the folloinng equations are summarized in the range of temperature of 308°〜309°K, (Vn)/(Cm) of 0.55〜0.66,and acid concentration of 0.9〜6.6.(Vi)/(Cm)=6.60-0.94 Ci (4) (Vm)/(Cm)=0.70-0.107 Ci (5) It will be seen from Fig. 8 that the effect of the feeding rate of corn steep liquor on the average specific rate of mycelial growth increased with the increase in the specific rate of feeding of corn steep liquor. However, the stimuratory effect of the feeding rate deceased with the increase of acid concentration in fermenting broth, and at 6.5% in acid concentration, no feeding effect on mycelial growth was observed.Substitution of the equation (4) in (1) gives the following : Vi=1.34・10^<19>・Cm・(6.60-0.94 Ci) exp {(-13540)/T} (6) Similarly, substituting (5) in (2) : Vm=8.26・10^<16>・Cm (0.70-0.107 Ci) exp {(-11230)/T} (7) where T=297.5°〜308°K, Cs=0.2〜7,Ci=0.9〜6.6,(Vn)/(Cm)=0.55〜0.66,Cm=0.5〜1.6,The correlation between the average specific rates of the acid production and the mycelial growth in single stage steady state fermentation is in contrast with that of batch one as shown in Fig. 9. It will be noted that the acid productivity was markedly dependent on the mycelial growth in single stage steady state continuous through flow fermentation of itaconic acid with Asp. terreus K 26 at a higher mycelial concentration than usual.
社団法人日本生物工学会の論文
1964-11-25

イタコン酸醗酵に関する研究 : (第5報)連続培養に関する基礎的研究 : (その3)単槽定常連続培養におけるイタコン酸集積速度, 菌体増殖速度におよぼす培養温度およびCSL供給速度の効果

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