キシロオリゴ糖の調製法

概要

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Hardwood xylan (800g as xylan) was partially hydrolyzed at 68_??_70°C with the xylanase system of Streptomyces sp. E-86. Candida guilliermondii IFO 0566 was aerobically grown in the resulting supernatant hydrolyzate (456g as anhydroxylose) supplemented with some nutrients, at 30°C for 19.5 hr. Only xylose and xylobiose were selectively metabolized by the yeast, and the oligomers higher than xylotriose left in the culture broth. After removal of yeast cells by centrifugation, the xylooligomers in the supernatant of culture broth (309g as anhydroxylose) were separated by chromatography on a carbon column, and the appropriate fractions were further purified by rechromatography on carbon columns. The main fractions were concentrated to yield crystalline products. The crystals were recrystallized from aqueous ethanol (yields; xylotriose of 46.4g, xylotetraose of 22.1g, xylopentaose of 15.3g, xylohexaose of 12.1g and a small amounts of xyloheptaose). Physical data of the xylooligomers prepared are summarized.
社団法人日本農芸化学会の論文