亜硫酸存在下のキシロースとフルフラールの定量

概要

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In connection with the investigation of hemicellulose hydrolysis, the determination method of xylose and furfural was studied. Influences of sulfurous acid and furfural on xylose determination by Somogyi's method could not be ignored (Fig. 1). Sulfurous acid could be removed by air oxidation in the presence of copper catalyst (Table 1), but the oxidation was disturbed by furfural (Table 2). The following procedure was recommended, by which xylose and furfural can be determined in the presence of sulfurous acid using the colorimetric method with a camphor-sulfuric acid reagent. Add 3 ml of camphor-sulfuric acid reagent to 2 ml of the sample solution and mix thoroughly. The absorbancy is measured immediately at 480 mμ, using 1-cm cell (Fig.2). The reading is made against distilled water. Convert the absorbancy reading to μg of furfural by the calibration curve I (Fig. 4). After heating the same solution in a boiling water bath for 20 min (Fig. 3), measure the absorbancy (A). Convert μg of furfural to the absorbancy reading after heating (A_F) by the calibration curve III (Fig. 4). Xylose is obtained by converting (A-A_F+0.2) using the calibrationcurve II (Fig. 4).
社団法人日本生物工学会の論文
1966-02-25