高温菌のチターゼについて : (第1報)Thermoactinomyces vulgarisによるチターゼ生産

概要

論文の詳細を見る
Thermophilic microorganisms were tested for the formation of cytase at 55°. Four hundred and seventy two strains having "Lysed zone" around the colonies were isolated using a "Barley medium" from 609 samples of soils and composts. Although all organisms tested had some cytase activity, several strains of Thermoactinomyces vulgaris gave the best results. For the determination of cytase activity, the method deviced by S. Kudo was applied, which is based on the decrease of turbidity in barly powder suspension. The supermatants of Thermoactinomyces vulgaris contained protease, α-amylase, pectinase, xylanase and β-glucanase activities. Moreover, these culture supernatants were capable of lysing slices of barley and yeast cell walls. The cultural conditions were investigated for cytase formation. Cytaseactivity could be precipitated with 2 volumes of ethyl alcohol (-16°) or 0.9 saturation of (NH_4)_2SO_4 from culture supernatants.
社団法人日本生物工学会の論文
1967-09-25