Trichoderma virideのキシラナーゼ : 酵素反応の一般的性質およびキシラン糖化の予備実験

概要

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In obtaining xylose from xylan, the authors were interested in comparing the biochemical process with the acid hydroysis process.Various kinds of moled were isolated from soil and screened out in reference to their xylanase actvity. It was found that Trichoderma viride N35 had a strong activity xylanase activity. Therefore, the present study on the process of xylanase production was conducted by using this strain. The enzymatic characteristics of the culture filtrate of Trichoderma viride were as follows : 1) The optimum pH for the saccharifying activity was found to be 4.2 and the optimum pH for the liquefying activity lay between 4.2 and 5.6. 2) The optimum temperature for the saccharifying activity was found to be 47℃ and the optimum temperature for the liquefying activity lay between 33℃ and 45℃.3) After the culture filtrate was allowed to stant at pH 4.2 and 50℃ for 1 and 2 hr., 96 and 83% of the saccharifying activities survived. The residual liquefying activities were 70 and 45% erspectively under the same conditions.4) 59.5 grams xylan was hydrolyzed by 700 ml of an enzymatic solution at pH 4.2 and 47℃ for 120 hrs. Xylan was completely liquefied. 675ml hydrolyzate sontaining 67.6 grams of total sugar were involved as xylose was obtained and its average polymerization grade was 1.06.
社団法人日本生物工学会の論文
1968-08-25