微生物によるレダクトン生成に関する研究 : (第1報)麹菌によるレダクトン様物質の生成と分画法について

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Production of reductones by mold Aspregilli has been studied by using 60 strains of Asp.isolated from miso and koji. Fifteen per cent glucose was used as a carbon source mixed with other mineral salts.Thirteen moulds decolorized over 30ml of 0.001M2,6-dichlorophenol indophenol (D.P.I) solution per 100ml broth. The broth was extracted with ethyl ether and the extract was paperchromatographically studied according to Michell by using D.P.I. as an indicator.Three kinds of gropus were observed, whose Rf values were (A) 0.53〜0.55,(B) 0.61〜0.64,and (C) 0.76〜0.77 respectively.When other reagents were used as indicators, ammonical silver nitrate reacted positively on all groups, acidic reaction using bromophenol blue was positive for (A) and (C), ferric chloride was positive for (B), while p-aminobenzoic acid did not react on all the three.Polarograms of the extract showed oxidation waves as those of ascorbic acid except (B). The maximum ultra violet absorption spectra (pH 2) were (A) 261mμ(B) 270mμ and (C) 265mμ respectively. Corresponding 2,4-dinitrophenyl-hydrazones were all deep purple colored in alcoholic potassium hydroxide soluiton.
社団法人日本生物工学会の論文
1964-05-15