シクロヘキサノン資化性菌の分離と培養条件

概要

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A cyclohexanone-utilizing bacterium was isolated from soil and identified as a spedies of the genus Pseudomonas from its morphological and biochemical characteristics.The optimal culture conditions of this organism were as follows : The growth medium contained cyclohexanone 0.1%, NH_4H_2PO_4 0.3%, KH_2PO_4 0.15%, Na_2HPO_4 0.15%, MgSO_4・7H_2O 0.02% CaCl_2・2H_2O 0.001%, FeSO_4・7H_2O 0.0005%, MnSO_4・4H_2O 0.0002% yeast extract 0.01%, pH 7.0. The culture was incubated at 30℃ for 2 days on a shaker.The isolate grew readily on cyclohexanone and cyclopentanone as cyclic ketones, and on o-hydroxybenzoic acid and p-hydroxybenzoic acid as aromatic compounds.This organism also grew on ε-caprolactone, 6-hydroxycaproate, and adipic acid, but not on 2-hydroxycyclohexanone.
社団法人日本生物工学会の論文
1977-03-25