Aspergillus cellulosaeの生産する乳酸菌溶菌酵素

概要

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By selection of fungi which produce lytic enzymes for lactic acid bacteria, Aspergillus fumigatus and A. cellulosae were found to show high lytic activities against the heat-treated cells of Lactobacillus sp. Af. The extracellular lytic enzyme produced by A. cellulosae lysed Gram-positive bacteria(L. sake, Bacillus sphaericus, B. subtilis, Micrococcus luteus, etc.)and also Gram-negative bacteria(Pseudomonas putida and Alcaligenes faecalis). Optimal pH and temperature for the lysis were 6.0 and 50℃, respectively. Lytic activity was completely lost after treatment at 70℃ for 15 min. Production of this enzyme was stimulated by the addition of xylose, yeast extract, meat extract, KH_2PO_4 and Na_2HPO_4 to the culture medium. The enzyme was purified about 21-fold by adsorption on Amberilite IRC-50 and precipitation by the addition of ammonium sulfate to 60% saturation. Partially-purified enzyme showed N-acetylhexosaminidase activity, but N-aetylglucosaminidase activity was not detected with the enzyme. A mixture of the partially-purified enzyme and a commercial lysozyme showed improved lysis of lactic acid bacteria and protoplast formation of L. sake, comparedd with the respective enzymes.
社団法人日本生物工学会の論文
1991-03-25