シクロヘキシル酢酸資化性菌の分離と培養条件

概要

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A buceterium, RCO-4M, that can grow on cyclohexylacetic acid as the sole carbon source was isolated from soil by enrichment cultures. The isolate was identified as a species of the genus Micrococcus by taxonomic studies.The potimal culture conditions of this organism were as follows : the growth medium consisted of cylohexylacetic acid 0.15%, KNO_3 0.25%, KH_2PO_4 0.1%, MgSO_4・7H_2O 0.002%, FeCl_3・6H_2O 0.005%, CaCl_2・2H_2O 0.002%, and yeast extract 0.01%, pH8.0.The culture was incubated at 30℃ for 3-7 days on a shaker.Strain RCO-4M grew on a number of types of alicyclic compounds. Among the alicyclic carboxylic acids tested, cyclohexylacetic acid and cyclohexylbutyric acid were the best substrates. The organism grew on cyclohexylpropionic acid, and grew a little on both cyclohexanecarboxylic acid and cyclopentanecarboxylic acid.This isolate also grew on cyclohexanone and cyclohexanol.Strain RCO-4M grew well on all of the n-alkylcyclohexanes having 6-14 carbon atoms in the n-alkyl group, after a long lag time.
1988-01-25