Tomato spotted wilt virusによるトマトの黄化えそ病

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Since 1972 an outbreak of a virus disease of tomato has been found in Nara Prefecture. The symptoms were characterized by yellowing and severe necrotic spots on leaves and necrotic streaks on petioles and stems. Necrotic symptoms were also observed on deformed fruits. By sap inoculation test 30 species in 8 families out of 36 species in 11 families were found to be susceptible to the causal virus. The symptoms observed were pronounced necrosis in many of the susceptible plants, especially of Solanaceae. In epidermal strips of infected tomato and Datura stramonium, cellular inclusions were easily observed. The virus was also transmitted by a thrips, Thrips tabaci, but not through infected tomato seeds. In crude sap prepared with 0.1M phosphate buffer (pH 7.0) or distilled water, the thermal inactivation point of the virus was between 45 and 50 C or 35 and 40 C, the dilution end point was between 10^<-5> and 2×10^<-5> or 10^<-4> and 2×10^<-4>, and the longevity in vitro was between 10 and 15 hr or 5 and 10 hr, respectively. Virus particles observed in leaf-dip preparation from diseased plants were found to be spherical, 70-90 nm in diameter, presenting an outer layer structure when fixed with OsO_4 vapor before negative staining with 2% PTA (pH 7.0). In unfixed preparations, most of the virus particles were deformed to various extents. In ultrathin sections of the infected leaf cells of tomato, clusters of spherical virus particles of 70-90 nm in diameter were frequently observed in cytoplasm enclosed in vesicles. From the above mentioned results, the causal virus was identified as tomato spotted wilt virus (TSWV). This is the first record of the occurrence of TSWV in tomato in Japan.
日本植物病理学会の論文
1976-07-25

Tomato spotted wilt virusによるトマトの黄化えそ病

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