イミュノキャプチャーPCRによるイチョウイモ(Dioscorea opposita Thunb. cv. ichoimo)からのヤマノイモモザイクウイルス(JYMV)の検出とそのPCR-RFLPによる強毒株と弱毒株の判別

概要

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Detection of Japanese yam mosaic potyvirus (JYMV) in Chinese yam (Dioscorea opposita Thunb. cv. ichoimo) by the immunocapture polymerase chain reaction (IC-PCR) and differentiation of virulent and attenuated isolates using restriction fragment length polymorphism (RFLP) of IC-PCR products using Tsp 509 I were investigated. JYMV was detected by IC-PCR from diseased leaves, bulbils, tubers or diseased leaves frozen at -40℃ for 6 months with a sensitivity of 10^8, 10^5, 10^6 and 10^7-fold dilutions, respectively. The sensitivity of IC-PCR for the detection of JYMV from diseased leaves was about 8000-fold over ELISA and 10,000-fold over RT-PCR. PCR-RFLP revealed different banding patterns for the virulent and attenuated isolates of JYMV. This will be useful for distinguishing virulent and attenuated isolates from Chinese yarn (cv. ichoimo).
日本植物病理学会の論文
1999-08-25