Effect of Temperature Change and Aeration on the Production of the Antifungal Peptide Antibiotic Iturin by Bacillus subtilis NB22 in Liquid Cultivation
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概要
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The effects of temperature and air supply on the production of the antifungal lipopeptide antibiotic, iturin by Bacillus subtilis NB22 in a 5-d course of liquid cultivation were investigated on a flask scale. Changing the temperature from 30℃ to 25,37,42,or 48℃ for one day during the 5-d cultivation reduced the amount of iturin producted in comparison with cultivation at a constant 30℃. The temperature on the first day was notably critical. When the air supply was varied by changing the plugs of the shaking flasks, the air supply was also critical on the first day. When cells spent the first day with an inappropriate temperature or air supply, the decrease in iturin production could not be compensated for during the subsequent 4-d cultivation at an adequate temperature and air supply. Although iturin production was significant on the second and third days when the growth of the bacterium had leveled off, the initial conditions severely affected the yield of the product.
- 公益社団法人日本生物工学会の論文
- 1993-06-25
著者
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正田 誠
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Ano T
Tokyo Inst. Technol. Yokohama Jpn
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Ano Takashi
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Shoda Makoto
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Shoda Makoto
Research Laboratory Of Resources Utiluzation Tokyo Institute Of Technology
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OHNO AKIHIRO
Research Laboratory of Resources Utilization, Tokyo Institute of Technology
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Ohno Akihiro
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Shoda Makoto
Research Lab. Resources Utilization Tokyo Institute Of Technology
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