Characteristics of Plasmid Stability in Bacillus subtilis NB22,an Antifungal-Antibiotic Iturin Producer
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概要
- 論文の詳細を見る
The stability of plasmids pC194 and pUB110 was investigated in an antifungal antibiotic iturin producer, Bacillus subtilis NB22. Although plasmid pC194 was maintained stably over a hundred generations in five succesive cultivations in iturin-production no. 3 medium, significant curing was observed when the cultivation was prolonges for 10 d in the same medium. When the transformant of pC194 was cultivated in Schaeffer's sporulation medium, drastic curing took place in accordance with the occurrence of sporulation, even in the presence of an antibiotic for selective pressure. In the case of pUB110,in sharp contrast to the result with pC194,high stability was observed in the sporulation medium, but significant curing was observed during prolonged incubation in no. 3 medium.
- 公益社団法人日本生物工学会の論文
- 1993-04-25
著者
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正田 誠
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Ano T
Tokyo Inst. Technol. Yokohama Jpn
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Ano Takashi
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Shoda Makoto
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Shoda Makoto
Research Laboratory Of Resources Utiluzation Tokyo Institute Of Technology
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TOKUDA YUKINORI
Research of Laboratory of Resources Utilization, Tokyo Institute of Technology
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Tokuda Yukinori
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Shoda Makoto
Research Lab. Resources Utilization Tokyo Institute Of Technology
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