High-Efficiency Transformation of Bacillus subtilis NB22,an Antifungal Antibiotic Iturin Producer, by Electroporation
スポンサーリンク
概要
- 論文の詳細を見る
High-voltage electroporation was used to transform Bacillus subtilis NB22,an antifungal antibiotic producer, reaching the efficiency of 10^7 transformants/μg plasmid DNA. Transformation frequency was dependent on the composition of the electroporation solution, the electrical field strength and the cell concentration. Addition of polyethylene glycol (PEG) and mannitol in the transformation solution was critical for a high efficiency of transformation.
- 公益社団法人日本生物工学会の論文
- 1992-04-25
著者
-
Ano Takashi
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
-
Shoda Makoto
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
-
MATSUNO YUKO
Research Laboratory of Resources Utilization, Tokyo Institute of Technology
-
Matsuno Yuko
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
-
Shoda Makoto
Research Lab. Resources Utilization Tokyo Institute Of Technology
-
SHODA MAKOTO
Research Laboratory of Resources Utilization, Tokyo Institute of Technology
関連論文
- A New Cultivation System Operated under a Super High Magnetic Field
- Nucleotide Sequence and Characteristics of the Gene, lpa-14,Responsible for Biosynthesis of the Lipopeptide Antibiotics Iturin A and Surfactin from Bacillus subtilis RB14
- Characteristics of H_2S Oxidizing Bacteria Inhabiting a Peat Biofilter
- Thermal Balance in Composting Operations
- Rate of Composting of Dewatered Sewage Sludge in Continuously Mixed Isothermal Reactor
- Bacterial Control of Plant Diseases
- Effect of Homogeneous and Inhomogeneous High Magnetic Fields on the Growth of Escherichia coli
- Effect of Super High Magnetic Field on the Growth of Escherichia coli under Various Medium Compositions and Temperatures
- DECOMPOSITION PROCESS OF VARIOUS ORGANIC WASTES IN SOIL WITH REFERENCE TO GEL CHROMATOGRAPHY
- Investigation of Optimal Conditions for Foam Separation of Iturin, an Antifungal Peptide Produces by Bacillus subtilis
- Effect of Molasses on the Production and Activity of Dye-Decolorizing Peroxidase from Geotrichum candidum Dec1
- cDNA Cloning and Genetic Analysis of a Novel Decolorizing Enzyme, Peroxidase Gene dyp from Geotrichum candidum Dec 1
- lpa-14, a Gene, Involved in the Production of Lipopeptide Antibiotics, Regulates the Production of a Siderophore, 2, 3-Dihydroxybenzoylglycine, in Bacillus subtilis RB14
- Batch Decolorization of Molasses by Suspended and Immobilized Fungus of Geotrichum candidum Dec 1
- Characteristics of Bacillus subtilis Isolated from Composts Suppressing Phytopathogenic Microorganisms
- Characteristics of a Newly Isolated Fungus, Geotrichum candidum Dec 1,Which Decolorizes Various Dyes
- Treatment of Exhaust Gases from a Night Soil Treatment Plant by a Combined Deodorization System of Activated Carbon Fabric Reactor and Peat Biofilter Inoculated with Thiobacillus thioparus DW44
- Removability of Malodorous Gases from a Night Soil Treatment Plant by a Pilot-Scale Peat Biofilter Inoculated with Thiobacillus thioparus DW44
- Enhanced Removability of Odorous Sulfur-Containing Gases by Mixed Cultures of Purified Bacteria from Peat Biofilters
- Enhanced Removal Efficiency of Malodorous Gases in a Pilot-Scale Peat Biofilter Inoculate with Thiobacillus thioparus DW44
- Degradation Characteristics of Hydrogen Sulfide, Methanethiol, Dimethyl Sulfide and Dimythyl Disulfide by Thiobacillus thioparus DW44 Isolated from Peat Biofilter
- Removal of Dimethyl Disulfide by the Peat Seeded with Night Soil Sludge
- Removal Characteristics of Hydrogen Sulphids and Methanethiol by Thiobacillus sp. Isolated from Peat in Biological Deodorization
- Method for Monitoring the Decomposition Process in Soil of Organic Wastewaters with High Strength and Low C/N Ratio
- Removal of a High Load of Ammonia Gas by a Marine Bacterium, Vibrio alginolyticus
- 556 Ammonia removability in peat biofilter seeded with enriched nitrifying bacteria isolated from night soil sludge.
- 809 Ammonia Removal by Peat Biofilter Seeded with Night Soil Activated Sludge.
- Persistence of Bacillus subtilis RB14 and Its Derivative Strains in Soil with Respect to the lpa-14 Gene
- Ultra-rapid Transformation of Escherichia coil by an Alkali Cation
- STABILIZATION PROCESS OF SEWAGE SLUDGE COMPOST IN SOIL
- HIGH PERFORMANCE SIZE EXCLUSION CHROMATOGRAPHY OF WATER EXTRACT FROM SEWAGE SLUDGE-SOIL MIXTURE
- FACTORS AFFECTING THE STABILIZATION PERIOD OF SEWAGE SLUDGE IN SOIL WITH REFERENCE TO THE GEL CHROMATOGRAPHIC PATTERN
- APPLICATION OF GEL CHROMATOGRAPHY TO MONITOR THE DECOMPOSITION PROCESS OF SEWAGE SLUDGE IN SOIL
- Suppressive Effect of Bacillus subtilis and It's Products on Phytopathogenic Microorganisms
- Nitrogen Transformation during Thermophilic Composting
- Oxygen Diffusion and Microbial Activity in the Composting of Dehydrated Sewage Sludge Cakes
- Effects of a Bulking Agent on the Reaction Rate of Thermophilic Sewage Sludge Composting
- Removal of NH_3 by a Peat Biofilter without and with Nitrifier
- Removal of Hydrogen Sulfide by a Biofilter with Fibrous Peat
- Removal Characteristics of Dimethyl Sulfide by a Mixture of Hyphomicrobium sp. I55 and Pseudomonas acidovorans DMR-11
- Removal Characteristics of Dimethyl Sulfide, Methanethiol and Hydrogen Sulfide by Hyphomicrobium sp. I55 Isolated from Peat Biofilter
- Effect of Temperature Change and Aeration on the Production of the Antifungal Peptide Antibiotic Iturin by Bacillus subtilis NB22 in Liquid Cultivation
- Characteristics of Plasmid Stability in Bacillus subtilis NB22,an Antifungal-Antibiotic Iturin Producer
- Survival of Bacillus subtilis NB22,an Antifungal-Antibiotic Iturin Producer, and Its Transformant in Soil-Systems
- Plasmid Instability in Bacillus subtilis during Sporulation
- Production of the Antifungal Peptide Antibiotic, Iturin by Bacillus subtilis NB22 in Solid State Fermentation
- Molecular Cloning of a Gene Responsible for the Biosynthesis of the Lipopeptide Antibiotics Iturin and Surfactin
- Rapid Transformation of Baciilus subtilis Using KCl-Treatment
- Suppressive Effect In Vitro of Magnesium L-Ascorbyl-2-phosphate (APM), an Ascorbic Acid Derivative, on the Impairment of DNA by Glucose-6-phosphate(Food & Nutrition)
- Effect of Temperature on Production of Lipopeptide Antibiotics, Iturin A and Surfactin by a Dual Producer, Bacillus subtilis RB14,in Solid-State Fermentation
- Constitutive Mutants for Dextransucrase from Leuconostoc mesente4roides NRRL B-512F
- High-Efficiency Transformation of Bacillus subtilis NB22,an Antifungal Antibiotic Iturin Producer, by Electroporation
- Expression of the Suppressive Effect of Badillus subtilis on Phytopathogens in Inoculated Composts
- Composting reaction Rate of Sewage Sludge in an Autothermal Packed Bed Reactor
- Biological Deodorization Using Activated Carbon Fabric as a Carrier of Microorganisms
- Effect of High Magnetic Field on Microbial Growth