Investigation of Optimal Conditions for Foam Separation of Iturin, an Antifungal Peptide Produces by Bacillus subtilis

概要

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An antifungal peptide, iturin, was produced by a newly-isolated Bacillus subtilis, which contains cyclic 7 D- and L-α amino β-amino acids with aliphatic side chains. It consists of 6 derivatives with different length side chains. When the bacterium was cultivated in a jar fermentor, all the iturin produced was entrained in the foam. Thus, continous separation and condensation of the product iturin is possible only by collecting the foam produced during fermentation. The controlling factors for foaming, namely the aeration rate, temperature and medium composition, especially the ratio of glucose to Polypepton, were investigated in relation to iturin production. The most effective conditions for the condensation of iturin were an aeration rate of 0.1 vvm, a temperature of 30℃, and a glucose to Polypepton ratio of 0.3-0.4. The total amount of iturin produced, and the ratio of peaks 3,4 and 5 in the iturin were maximized at 50 g/l of Polypepton by using a fermentor equipped with a basket-shaped agitation unit.
社団法人日本生物工学会の論文
1991-02-25