Nucleotide Sequence and Characteristics of the Gene, lpa-14,Responsible for Biosynthesis of the Lipopeptide Antibiotics Iturin A and Surfactin from Bacillus subtilis RB14

概要

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Bacillus subtillis RB14 is a coproducer of the lipopeptide antibiotics iturin A and surfactin. Iturin A and surfactin have a simillar structure consisting of a seven-amino-acid cyclic peptide, linked by either hydroxy or ester peptide linkage, respectively, to the fatty acid part. A 10-kb fragement responsible for the production of the lipopeptide antibiotics iturin A and surfactin in B. substilis RB14 was minimized and the nucleotide sequence of the region essential for the synthesis of the lipopeptides was determined. A large open reading frame consisting of 224 amino acid residues was found. The gene, designated as lpa-14 (lipopeptide antibiotic production of RB14), showed high homology with sfp and psf-1 (regulators for surfactin production in B. subtilis and Bacillus pumilus, respectively) and an unknown open reading frame, orfX, in the upstream region of the peptide antibiotic gramicidin S biosynthesis operon of Bacillus brevis. The biosynthesis of surfactin and of iturin A was shown to be coregulated by the same gene, lpa-14. It is suggested that a common regulation system might exist amoung these genes for the productin of peptide antibiotics in Bacillus species.
社団法人日本生物工学会の論文
1993-12-25