Constitutive Mutants for Dextransucrase from Leuconostoc mesente4roides NRRL B-512F

概要

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Constitutive mutants for dextransucrase were isolated from cellus of Leuconostoc mesenteroides NRRL B-512F by treatment with N-methyl-N'-nitro-N-nitroguanidine, growing on an agar containing glucose as a carbon source and overlaying a soft agar with sucrose and tetracycline. These mutants were able to produce the enzyme in a liquid media containing sugars other than sucrose, such as glucose, fructose and maltose, without simultaneous synthesis of dextran. The enzyme activity of one mutant strain, SH 3002,was 2- to 3-fold higher than that of the wild strain grown on sucrose. When the concentration of glucose in the medium was increased from 2 to 4%, a 1.7-fold increase of enzyme activity was obtained for the mutant, wheres only a slight increased of the activity was observed on sucrose for borh the wild strain and the mutant.
公益社団法人日本生物工学会の論文
1994-03-25