97 暗所芽生えにおいて細胞分裂及び色素体分化を調節するブラシノステロイドとサイトカイニンの作用について

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Brassinosteroid-deficient plants show characteristics of de-etiolated plants, i.e., short hypocotyls, expanded cotyledons and development of true leaves, when grown in darkness. In contrast, it is known that the application of cytokinin induces de-etiolated phenotype in the dark. In this context, brassinosteroid and cytokinin seem to exhibit opposite roles in photomorphogenesis. Here, we observed cell division and plastid development of dark-grown plants of brassinosteroid-deficiency and cytokinin-excess. Four-days-grown cyclin : : GUS transgenic Arabidopsis showed no GUS-staining in the dark. In contrast, Brz (an specific brassinosteroid biosynthesis inhibitor)-treated or benzyladenine (BA)-treated cyclin : : GUS plants showed intense GUS expression in shoot apex. In Brz plus BA-treated plants, additive GUS expression was observed. These results suggest that both brassinosteroid-deficiency and cytokinin-excess function cell division in development of true leaves in the dark. Brz-treated or BA-treated plants exhibited fast greening after transferring from dark to light. In Brz plus BA-treated plants, however, the fast greening effect was not additive. Transmission electron microscopical observation revealed that plastids of Brz-treated plants were etioplasts containing large prolammellar bodies, while those of BA-treated plants were young chloroplasts containing monolayer thylakoid membranes. Thus, brassinosteroid-deficiency and cytokinin-excess exhibited different effects in plastid development in the dark.
植物化学調節学会の論文
2002-10-31

97 暗所芽生えにおいて細胞分裂及び色素体分化を調節するブラシノステロイドとサイトカイニンの作用について

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