COMPLEMENTARY IMPROVEMENT OF THE METHOD FOR DETERMINING CHOLINERGIC ACTIVITIES IN THE SMALL INTESTINE AND ITS APPLICATION TO EXPERIMENTS IN VIVO
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概要
- 論文の詳細を見る
The concentration of acetylcholine (ACh) in rat jejunum that had been homogenized with an ultra-high-speed homogenizer (Biotron^<TM>) was significantly higher than that in jejunum homogenized with a glass homogenizer. Rats were injected once or repeatedly for 10 days with a muscarinic agonist, pilocarpine (1 mg/kg), or a muscarinic antagonist, scopolamine (5 mg/kg). Animals were killed 20 min or 24 hr after single or consecutive injections, respectively, for determinations of cholinergic activities in the jejunum. Single treatment: Pilocarpine did not cause significant changes in the level of ACh, the activity of acetylcholinesterase (AChE), the binding of [^3H] quinuclidinyl benzilate (QNB) or the contractile responses to ACh. Scopolamine reduced the level of ACh and binding of [^3H]QNB without inducing significant changes in the activity of AChE and the contractile response. Consecutive treatment: Pilocarpine reduced the binding of [^3H]QNB by changing the value of Bmax and reduced the contractile response without affecting the level of ACh or the activity of AChE. Scopolamine increased the binding of [^3H]QNB without any effects on the level of ACh, the activity of AChE or the contractile response. In summary, it is possible to determine the level of ACh in a tissue as hard as intestine by homogenization with a Biotron^<TM> and to assess the cholinergic situation in the intestine of animals that have been poisoned with various agents by estimating cholinergic activities.
- 日本トキシコロジー学会の論文
- 1994-08-25
著者
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Sato Itaru
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
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KOBAYASHI Haruo
Department of Electronic Engineering, Graduate School of Engineering, Gunma University
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Matsusaka N
Iwate Univ. Morioka Jpn
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Sato I
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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佐藤 格
東京理科大理学部応用化学科
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Sato Itaru
Department Of Applied Chemistry Faculty Of Science Science University Of Tokyo
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SUZUKI Tadahiko
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
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Sato I
Lab. Of Veterinary Public Health Dep. Of Veterinary Medicine Fac. Of Agriculture Iwate Univ.
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MATSUSAKA Naonori
Departments of Veterinary Pharmacology and Veterinary Public Health, Iwate University,
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Kobayashi Haruo
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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Kobayashi Haruo
Department Of Electronic Engineering Faculty Of Engineering Gunma University
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FUJII Shin-Ichiro
Ninohe Health Center
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AKATSU Yasuhiko
Fukushima Health Center
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Yuyama Akira
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
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Kobayashi Hiroko
Environmental Science Team Research Institute Of Japan Plant Protection Association
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Yuyama Akira
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
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Suzuki Tsuyoshi
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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Matsusaka Naonori
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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Suzuki Tadahiko
Department Of Surgery Osaka City University Medical School
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Yuyama A
Department Of Veterinary Medicine Faculty Of Agriculture Iwate University
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Suzuki Tadahiko
Department Of Veterinary Pharmacology Faculty Fo Agriculture Iwate University
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Matsusaka Naonori
Faculty Of Agriculture Iwate University
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KOBAYASHI HARUO
Department of Animal Breeding, Faculty of Agriculture, University of Tokyo
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