Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius
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概要
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We isolated a cellulase from the digestive organs of the short-spined sea urchin Strogylocentrotus intermedius using a combination of ion-exchange chromatography and gel filtration together with an assay for carboxymethylcellulase activity. The isolated cellulase was stained as a single band by Congo red. The molecular weight of the isolated cellulase, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, was 59 kDa. The isolated cellulase exhibited hydrolytic activity toward carboxymethyl cellulose, with an optimum temperature and pH of 30℃ and pH 8.0, respectively. The thermal stability of the enzyme was characterized by determining the temperature at which activity decreased by 50 % with treatment for 30 min at pH 7.0 and found to be 32℃. Cellulase activity remained at a high level at 5-20℃, which is the growth temperature of the short-spined sea urchin. These results confirm that the short-spined sea urchin should preferably be reared at a water temperature of < 20℃.
- 2012-09-01
著者
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Ura Kazuhiro
Graduate School Of Fisheries Science Hokkaido University
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Takagi Yasuaki
Laboratory Of Aquatic Food Science Department Of Fisheries Faculty Of Agriculture Kinki University
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Ojima Takao
Laboratory Of Biochemistry And Biotechnology Graduate School Of Fisheries Sciences Hokkaido Universi
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Tanaka Hiroyuki
Laboratory Of Bioresponses And Regulation United Graduate School Of Drug Discovery And Medical Infor
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HASEGAWA Syuto
Laboratory of Comparative Physiology, Graduate School of Fisheries Science, Hokkaido University
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Hasegawa Syuto
Laboratory Of Comparative Physiology Graduate School Of Fisheries Science Hokkaido University
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Tanaka Hiroyuki
Laboratory of Marine Molecular Biology, Graduate School of Fisheries Science, Hokkaido University
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