Comparative study on general properties of alginate lyases from some marine gastropod mollusks

スポンサーリンク

概要

• 論文の詳細を見る

• Alginate lyase (EC 4.2.2.3) is an enzyme that splits glycosyl linkages of alginate chain via β-elimination producing unsaturated oligoalginates. This enzyme is widely distributed in herbivorous marine mollusks, brown algae, and marine and soil bacteria. In the present study, we determined the general properties and partial amino-acid sequences of alginate lyases from three Archeogastropoda, i.e., Haliotis discus hannai, H. iris, and Omphalius rusticus, and one Mesogastropoda, i.e., Littorina brevicula, in order to enrich the information about functional and structural diversity in gastropod alginate lyases. The alginate lyases were extracted from hepatopancreas of these animals and purified by ammonium sulfate fractionation followed by conventional column chromatography. Single alginate lyases with molecular masses of approximately 28 kDa, 34 kDa, and 34 kDa were isolated from H. discus, H. iris, and O. rusticus, respectively. While three alginate lyases with molecular masses of 35 kDa, 32 kDa, and 28 kDa were isolated from L. brevicula. These enzymes were identified as poly(M) lyase (EC 4.2.2.3) since they preferably degraded poly(M)-rich substrate. Western blot analysis using an antiserum raised against H. discus enzyme suggested that H. iris, and O. rusticus enzymes shared similar primary/higher order structure with H. discus enzyme, but the L. brevicula enzymes did not. H. discus, H. iris, and O. rusticus enzymes were classified to polysaccharide-lyase family-14 by the analysis of partial amino-acid sequences, while the L. brevicula enzymes were not.

• Springer Japanの論文
• 2009-05-01

著者

• Ojima Takao

  Lab. Of Marine Biotechnology And Microbiology Graduate School Of Fisheries Sciences Hokkaido Univ. H

• HATA Mami

  Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido

• KUMAGAI Yuya

  Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido

• RAHMAN Mohammad

  Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido

• CHIBA Satoru

  Central Research Laboratory, Nippon Suisan Kaisha Ltd.

• TANAKA Hiroyuki

  Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido

• INOUE Akira

  Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido

• OJIMA Takao

  Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido

• Hata Mami

  Laboratory Of Marine Biotechnology And Microbiology Graduate School Of Fisheries Sciences Hokkaido U

• Hata Mami

  Laboratory Of Experimental Radiology Aichi Cancer Center Research Institute

• Ojima Takao

  Laboratory Of Biochemistry And Biotechnology Graduate School Of Fisheries Sciences Hokkaido Universi

• Kumagai Yuya

  Lab. Of Marine Biotechnology And Microbiology Graduate School Of Fisheries Sciences Hokkaido Univ. H

• Chiba Satoru

  Central Research Laboratory Nippon Suisan Kaisha Ltd.

• Chiba Satoru

  Central Research Laboratory Nippon Suisan Corporation

• Kumagai Yuya

  Laboratory Of Marine Biotechnology And Microbiology Graduate School Of Fisheries Sciences Hokkaido U

• Rahman Mohammad

  Laboratory Of Function And Morphology Department Of Animal Science Faculty Of Agriculture Utsunomiya

• Tanaka Hiroyuki

  Laboratory Of Bioresponses And Regulation United Graduate School Of Drug Discovery And Medical Infor

• Rahman Mohammad

  Laboratory Of Marine Biotechnology And Microbiology Graduate School Of Fisheries Sciences Hokkaido University

• Inoue Akira

  Laboratory Of Marine Biotechnology And Microbiology Graduate School Of Fisheries Sciences Hokkaido University

• Rahman Mohammad

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, Sendai 980-8577, Japan

• Inoue Akira

  Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries Sciences, Hokkaido University

• Rahman Mohammad

  Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan

関連論文

• An endo-β-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai
• Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai
• Comparative study on general properties of alginate lyases from some marine gastropod mollusks
• Nafamostat Mesilate, a Potent Serine Protease Inhibitor, Inhibits Airway Eosinophilic Inflammation and Airway Epithelial Remodeling in a Murine Model of Allergic Asthma
• A Novel CC-Chemokine Receptor 3 Antagonist, Ki19003, Inhibits Airway Eosinophilia and Subepithelial/Peribronchial Fibrosis Induced by Repeated Antigen Challenge in Mice
• Effect of Diesel Exhaust Particles on House Dust Mite–Induced Airway Eosinophilic Inflammation and Remodeling in Mice
• Lactobacillus Acidophilus Strain L-92 Regulates the Production of Th1 Cytokine as well as Th2 Cytokines
• Production of Matrix Metalloproteinases in Human Cultured Mast Cells : Involvement of Protein Kinase C-Mitogen Activated Protein Kinase Kinase-Extracellular Signal-regulated Kinase Pathway
• Establishment of Allergic Dermatitis in NC/Nga Mice as a Model for Severe Atopic Dermatitis(Pharmacology)
• The Effect of a Newly Synthesized Indazole Compound, TAS-3-124, on Experimental Autoimmune Disease(Pharmacology)
• Role of T cells in IgE-dependent triphasic cutaneous reaction caused by dinitrofluorobenzene in the mouse ear : Participation of CD8^+ T cells
• P-53 Effects of Vaticanol (B, C and G), α-Viniferin and Hopeaphenol on the Release of Mediators from Bone Marrow-Derived Mast Cells
• Role of mast cells in antigen-induced airway inflammation and bronchial hyperresponsiveness in rats.
• Effect of a Novel Anti-allergic Agent, HSR-609,on Antigen-induced Airway Hyperresponsiveness in Mice.
• VUF-K-8788,a periphery-selective histamine H1 antagonist with anti-pruritic activities.
• Effect of Antiallergic Drugs on Interleukin 5-Induced Eosinophil Infiltration of Rat Airways
• Effects of Salmeterol Xinafoate and Fluticasone Propionate on Immunological Activation of Human Cultured Mast Cells
• N-Terminal Modification and Its Effect on the Biochemical Characteristics of Akazara Scallop Tropomyosins Expressed in Escherichia coli
• The effect of allergen-induced airway inflammation on airway remodeling in a murine model of allergic asthma.
• The effects of leukotrien D4 inhalation on the antigen-induced airway hyperresponsiveness and inflammation in 5-lipoxygenase gene-deficient mice.
• Disruption of antigen-induced airway inflammation and airway hyper-responsiveness in low affinity neurotrophin receptor p75 gene deficient mice.
• Preparation of β-1,3-glucanase from scallop mid-gut gland drips and its use for production of novel heterooligosaccharides
• Interleukin-4 Is Involved in Allergen-induced Airway Eosinophilic Inflammation and Bronchial Hyperresponsiveness Independent of Genetic Background.
• Chromosome evolution among seven Fagopyrum species revealed by fluorescence in situ hybridization (FISH) probed with rDNAs
• Pollen Tube Growth in Cross Combinations between Torenia fournieri and Fourteen Related Species
• Evolution of chromosomes in the genus Pennisetum
• Cysteinyl leukotrienes do not mediated lipopolysaccharide-induced airway hyperresponsiveness in guinea pigs.
• Effect of Byakko-ka-ninjin-to on experimental allergic cutaneous reaction
• Effects of cAMP-phosphodiesterase Isozyme Inhibitor on Cytokine Production by Lipopolysaccharide-stimulated Human Peripheral Blood Mononuclear Cells.
• Effects of SA13353, a Transient Receptor Potential Vanilloid 1 Agonist, on Leukocyte Infiltration in Lipopolysaccharide-Induced Acute Lung Injury and Ovalbumin-Induced Allergic Airway Inflammation
• Effect of repeated antigen inhalation on airway inflammation and bronchial responsiveness to acetylcholine in interleukin-5 transgenic mice.
• Identification of wheat alien chromosome addition lines for breeding wheat with high phosphorus efficiency
• Exploration of Triticeae seed storage proteins for improvement of wheat end-product quality
• Preferential elimination of chromosome 1D from homoeologous group-1 alien addition lines in hexaploid wheat
• Diversity of Low-Molecular-Weight Glutenin Subunit Genes in Asian Common Wheat (Triticum aestivum L.)
• Genetic Diversity and Species Relationship of Some Terrestrial Bladderwort (Utricularia L.) as Revealed by Inter Simple Sequence Repeat (ISSR) Markers
• Effect of Anti-IL-4 and Anti-IL-5 Antibodies on Allergic Airway Hyperresponsiveness in Mice.
• Effect of Isoenzyme Selective Phosphodiesterase Inhibitors on Bacterial Lipopolysaccharide-induced Bronchial Hyperreactivity in Guinea Pigs.
• Interferon-beta Prevents Antigen-induced Bronchial Inflammation and Airway Hyperreactivity in Mice.
• The Effect of Anti-IL-4 Monoclonal Antibody, Rapamycin and Interferon-γ on Airway Hyperreactivity to Acetylcholine in Mice.
• Participation of Leukotriene D4 and Tumor Necrosis Factor on Lipopolysaccharide-induced Airway Hyperresponsiveness in Guinea Pigs.
• Role of mast cells, eosinophils and IL-5 in the development of airway hyperresponsiveness in sensitized mice.
• Production of wheat-Psathyrostachys huashanica chromosome addition lines
• The Raf/MAP Kinase Cascade in Cell Cycle Regulation and Differentiation in Drosophila
• cDNA cloning and bacterial expression of an endo-β-1,4-mannanase, AkMan, from Aplysia kurodai
• Heat-induced structural changes and aggregation of walleye pollack myosin in the light meromyosin region
• Kojistatin A, a New Cysteine Protease Inhibitor Produced by Aspergillus oryzae
• Suppressive Effects of Tranilast on Pulmonary Fibrosis and Activation of Alveolar Macrophages in Mice Treated with Bleomycin: Role of Alveolar Macrophages in the Fibrosis
• cDNA cloning of an alginate lyase from a marine gastropod Aplysia kurodai and assessment of catalytically important residues of this enzyme.
• Effect of Porous Counter Electrode with Highly Conductive Layer on Dye-Sensitized Solar Cells
• Rapid Detection of Acetic Acid Bacteria in the Traditional Pot-Fermented Rice Vinegar Kurozu
• Effects of SA13353, a Transient Receptor Potential Vanilloid 1 Agonist, on Leukocyte Infiltration in Lipopolysaccharide-Induced Acute Lung Injury and Ovalbumin-Induced Allergic Airway Inflammation
• Regional specialization of the Ganglion cell density in the retina of the Ostrich (Struthio camelus)
• Concentrations of ketone body and antidiuretic hormone in cerebrospinal fluid in response to the intra-ruminal administration of butyrate in suckling calves
• Formation of Porous Titanium Film and Its Application to Counter Electrode for Dye-Sensitized Solar Cell
• Extended Application of Barley EST Markers for the Analysis of Alien Chromosomes Added to Wheat Genetic Background
• Participation of Leukotriene D_4 and Tumor Necrosis Factor on Lipopolysaccharide-Induced Airway Hyperresponsiveness in Guinea Pigs
• Genomic differentiation of Hordeum chilense from H. vulgare as revealed by repetitive and EST sequences
• Pathological Features of Salivary Gland Cysts in a Shiba Dog with GM1 Gangliosidosis: A Possible Misdiagnosis as Malignancy
• Orally supplemented Lactobacillus acidophilus strain L-92 inhibits passive and active cutaneous anaphylaxis as well as 2,4-dinitroflurobenzene and mite fecal antigen induced atopic dermatitis-like skin lesions in mice
• Production of wheat-Psathyrostachys huashanica chromosome addition lines
• A Novel CC-Chemokine Receptor 3 Antagonist, Ki19003, Inhibits Airway Eosinophilia and Subepithelial/Peribronchial Fibrosis Induced by Repeated Antigen Challenge in Mice
• Effect of Diesel Exhaust Particles on House Dust Mite-Induced Airway Eosinophilic Inflammation and Remodeling in Mice
• Characterization of a β-D-mannosidase from a marine gastropod, Aplysia kurodai
• Applicability of Aegilops tauschii drought tolerance traits to breeding of hexaploid wheat
• Transient Receptor Potential Vanilloid 1-a Polymodal Nociceptive Receptor-Plays a Crucial Role in Formaldehyde-Induced Skin Inflammation in Mice
• cDNA cloning and bacterial expression of a PL-14 alginate lyase from a herbivorous marine snail Littorina brevicula
• Heat-stability and primary structure of the major alginate lyase isozyme LbAly35 from Littorina brevicula
• Effects of the cytidine analogue zebularine on wheat mitotic chromosomes
• Development of diversity array technology (DArT) markers for assessment of population structure and diversity in Aegilops tauschii
• A simple method of chromosome doubling using colchicine in Torenia (Linderniaceae), and the behavior of meiotic chromosomes in amphidiploids
• Lactobacillus acidophilus Strain L-92 Induces CD4+CD25+Foxp3+ Regulatory T Cells and Suppresses Allergic Contact Dermatitis
• Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius
• Homology of two alien chromosomes during meiosis in wheat
• Identification of Bangladeshi Domestic Cats with GM1 Gangliosidosis Caused by the c.1448G>C Mutation of the Feline GLB1 Gene: Case Study
• E2-10 Oral Nigella Sativa Oil Ameliorates Ovalbumin-Induced Bronchial Asthma in Mice(English Session 2)
• Genotyping Assays for the Canine Degenerative Myelopathy-Associated c.118G>A (p.E40K) Mutation of the SOD1 Gene Using Conventional and Real-Time PCR Methods: A High Prevalence in the Pembroke Welsh Corgi Breed in Japan
• Real-Time PCR Genotyping Assay for GM2 Gangliosidosis Variant 0 in Toy Poodles and the Mutant Allele Frequency in Japan
• Homology of two alien chromosomes during meiosis in wheat

もっと見る 閉じる

スポンサーリンク