Extended Application of Barley EST Markers for the Analysis of Alien Chromosomes Added to Wheat Genetic Background

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概要

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• The main objective of this study is to produce DNA markers of polymorphism between wheat and some important alien species. The applicability of 1,165 barley EST primer sets to amplify markers showing polymorphism between wheat and ten alien species, covering a wide range of variation in Triticeae, was investigated. These primers consisted of four series. Series 1 was randomly chosen from a pool of barley EST primer sets. The remaining three were pre-screened in a previous study and showed polymorphic and coamplified patterns between barley and wheat. From 22% to 100% of the primer sets amplified single clear bands in the species; from 29% to 75% of which were polymorphic to wheat. The frequency of amplification corresponded with their phylogenetic distance from barley. Many DNA markers (78-859) showing polymorphism between each species and wheat were obtained. These markers are expected to be valuable in identifying the alien chromosomes added to a wheat genetic background. The usefulness of the markers in the basic and the applied studies of the wild species is discussed.

• 日本育種学会の論文

著者

• TANAKA Hiroyuki

  Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido

• Tsujimoto Hisashi

  Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University

• Tsujimoto Hisashi

  Laboratory Of Plant Genetics And Breeding Science Faculty Of Agriculture Tottori University

• Tsujimoto Hisashi

  Lab. Of Plant Genetics And Breeding Sci. Fac. Of Agriculture Tottori Univ.

• Sato Kazuhiro

  Barley And Wild Plant Resource Center Inst. Of Plant Sci. And Resources Okayama Univ.

• Sato Kazuhiro

  Research Center Energy And Environmental Technology Division Takuma Co. Ltd.

• Kishii Masahiro

  Kihara Institute For Biological Research Yokohama City University

• Tsujimoto Hisashi

  Laboratory Of Plant Genetics And Breeding Faculty Of Agriculture Tottori University

• Adel Abdel-Aziz

  Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University

• Kishii Masahiro

  Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University

• Adel Abdel-aziz

  Laboratory Of Plant Genetics And Breeding Science Faculty Of Agriculture Tottori University

• Tanaka Hiroyuki

  Laboratory Of Plant Genetics And Breeding Science Faculty Of Agriculture Tottori University

• Tanaka Hiroyuki

  Laboratory Of Bioresponses And Regulation United Graduate School Of Drug Discovery And Medical Infor

• Tanaka Hiroyuki

  Lab. Of Plant Genetics Fac. Of Agriculture Tottori Univ.

• Tanaka Hiroyuki

  Laboratory Of Plant Genetics Faculty Of Agriculture Tottori University

• Tsujimoto Hisashi

  Laboratory Of Molecular Breeding And Land Research Center Tottori University

• Hagras Abel

  Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University

• TSUJIMOTO Hisashi

  Laboratory of Genetics, Faculty of Agriculture, Kobe University

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