Barley EST Markers Enhance Map Saturation and QTL Mapping in Diploid Wheat
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概要
- 論文の詳細を見る
A linkage map was constructed using PCR-based markers developed from barley expressed sequence tags (ESTs) for recombinant inbred (RI) lines (F_10) derived from a cross between Triticum monococcum L. strain KT3-5 and T. boeoticum Boiss. strain KT1-1. Among the primer sets of 2,695 barley ESTs, 242 EST markers were integrated into the frame of a previously constructed diploid wheat linkage map with 96 RFLP markers. The total map length comprising barley EST, previously mapped RFLP and morphological markers was 1,038.1 cM, and the average distance between two adjacent loci was 3.1 cM. The linkage map showed saturation with the barley EST markers by covering extended telomeric regions and increasing the resolution compared with the previous RFLP map. Order of barley ESTs on the diploid wheat linkage map mostly showed a collinearity to that on the barley linkage map constructed previously, although the long arms of the homoeologous linkage groups 4 and 5 showed structural changes between diploid wheat and barley. Phenotypes of nine quantitative traits were scored for the diploid wheat RI lines and their parents. Composite interval mapping enabled to detect 24 quantitative trait loci for the traits. Thus, barley ESTs could become powerful tools to reveal homoeologous relationships between barley and wheat chromosomes, and valuable resources to develop genetic markers for wheat genomes.
- 日本育種学会の論文
著者
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SASAKUMA Tetsuo
Kihara Inst. Biol. Res., Yokohama City Univ.
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TAKEDA Kazuyoshi
Research Institute for Bioresources, Okayama University
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Sato Kazuhiro
Research Institute for Bioresources, Okayama University
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Sasakuma Tetsuo
Kihara Inst. Biol. Res. Yokohama City Univ.
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Sasakuma Tetsuo
Kihara Institute For Biological Research Yokohama City Universit
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Hori Kiyosumi
Research Institute For Bioresources Okayama University
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Sato Kazuhiro
Research Center Energy And Environmental Technology Division Takuma Co. Ltd.
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Takehara Sayuri
Research Institute for Bioresources, Okayama University
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Nankaku Nami
Research Institute for Bioresources, Okayama University
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Nankaku Nami
Research Institute For Bioresources Okayama University
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Takehara Sayuri
Research Institute For Bioresources Okayama University
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Takeda Kazuyoshi
Research Institute For Bioresources Okayama University
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Takeda Kazuyoshi
Research Institute Bioresource Okayama University
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