Dissection of barley chromosome 3H in common wheat and a comparison of 3H physical and genetic maps
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概要
- 論文の詳細を見る
We used the gametocidal system to dissect a barley chromosome 3H added to common wheat. The gametocidal system induced chromosomal structural changes in the 3H addition line of common wheat, and we cytologically screened for rearranged chromosomes involving the 3H chromosome by in situ hybridization (FISH/GISH). We established 50 common wheat lines carrying single rearranged (or dissected) 3H chromosomes of independent origin. The dissected 3H chromosomes were either deletions or translocations with wheat chromosomes, and their breakpoints were in the centromere/the long arm/the short arm in a rough ratio of 1:2:2. We used these so-called 3H dissection lines to map 36 EST markers that were polymorphic between euploid common wheat and the 3H addition line and that had been used for the construction of a 3H genetic map. We conducted PCR analysis to detect the EST markers in the dissection lines. The results of the PCR analysis, which mostly corresponded to the retained or lost segments of the dissected 3H chromosomes, allowed us to place the 36 EST markers into 20 chromosomal regions flanked by the breakpoints of the dissected chromosomes. We compared this physical map constructed in this study with a 3H genetic map constructed using the same EST markers. The order of all EST markers was consistent between the two maps. We briefly discuss on the advantage of the physical mapping using dissection lines over genetic mapping.
- 日本遺伝学会の論文
- 2009-02-25
著者
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Sato Kazuhiro
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Sato Kazuhiro
Research Institute for Bioresources, Okayama University
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Endo Takashi
Lab. Of Plant Genetics Graduate School Of Agriculture Kyoto Univ.
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NASUDA Shuhei
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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ENDO Takashi
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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Nasuda Shuhei
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Nasuda Shuhei
Lab. Of Plant Genetics Graduate School Of Agriculture Kyoto Univ.
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SAKAI Kazuhiko
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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Sato Kazuhiro
Research Center Energy And Environmental Technology Division Takuma Co. Ltd.
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Endo Takashi
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Endo Takashi
Laboratory Of Genetics Graduate School Of Agriculture Kyoto University
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