Chromosomal assignment and deletion mapping of barley EST markers
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概要
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From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.
- 日本遺伝学会の論文
- 2005-10-25
著者
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Islam A.
Department Of Anatomy And Cell Biology Graduate School Of Medical Sciences Kyushu University
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Sato Kazuhiro
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Sato Kazuhiro
Research Institute for Bioresources, Okayama University
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Endo Takashi
Lab. Of Plant Genetics Graduate School Of Agriculture Kyoto Univ.
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NASUDA Shuhei
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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ENDO Takashi
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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Nasuda Shuhei
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Islam A.
School Of Agriculture Food And Wine The Univ. Of Adelaide
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Nasuda Shuhei
Lab. Of Plant Genetics Graduate School Of Agriculture Kyoto Univ.
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ASHIDA Taizo
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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KIKKAWA Yukari
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University
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Sato Kazuhiro
Research Center Energy And Environmental Technology Division Takuma Co. Ltd.
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Kikkawa Yukari
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Ashida Taizo
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Islam A.
Department Of Agronomy Waite Agricultural Research Institute University Of Adelaide
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Endo Takashi
Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University
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Endo Takashi
Laboratory Of Genetics Graduate School Of Agriculture Kyoto University
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