Detection of terminal deletions in barley chromosomes by the PCR-based method

スポンサーリンク

概要

• 論文の詳細を見る

• Barley chromosomes have barley-specific repetitive sequences (HvT01) in the subtelomeric regions. The subtelomeric repeats were amplified by the polymerase chain reaction (PCR) in 11 wheat-barley telosome addition lines, but no or little amplification occurred in the euploid common wheat cultivar `Chinese Spring. A gametocidal chromosome 2C, derived from an Aegilops cylindrica chromosome, induces chromosome breaks in Chinese Spring. To explore the potential of using PCR as a screen for barley telosomes with terminal deletions, induced by chromosome 2C, the progeny of a barley ditelosomic (6HS) addition line of Chinese Spring carrying chromosome 2C was investigated. The progeny plants in which DNA amplification occurred by PCR always had a normal 6HS whereas those in which no DNA amplification was observed carried either no 6HS or a 6HS telosome with a deletion. In this work the PCR based assay described is shown to be a robust and reliable method of identifying terminal deletions in barley telosomes in a Chinese Spring background.

• 日本遺伝学会の論文

著者

• Nasuda Shuhei

  Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University

• Nasuda S

  Kyoto Univ. Kyoto Jpn

• YOSHINO Mitsuaki

  Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University

• R. ENDO

  Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University

• Yoshino Mitsuaki

  Laboratory Of Plant Genetics Graduate School Of Agriculture Kyoto University

• Endo Takashi

  Laboratory Of Genetics Graduate School Of Agriculture Kyoto University

関連論文

• Dissection of barley chromosome 4H in common wheat by the gametocidal system and cytological mapping of chromosome 4H with EST markers
• ロボットの遠隔操作のための環境構築
• Cytological dissection and molecular characterization of chromosome 1R derived from 'Burgas 2' common wheat
• NBRP, National Bioresource Project of Japan and plant bioresource management
• Identification of 205 current rice cultivars in Japan by dot-blot-SNP analysis
• Dissection of barley chromosome 3H in common wheat and a comparison of 3H physical and genetic maps
• Dissection of barley chromosome 5H in common wheat
• Chromosomal assignment and deletion mapping of barley EST markers
• Restoration of Seed Germination at Supraoptimal Temperatures by Fluridone, an Inhibitor of Abscisic Acid Biosynthesis
• A PCR-based marker for targeting small rye segments in wheat background
• Barley chromosome addition lines of wheat for screening of AFLP markers on barley chromosomes
• Chromosome-specific satellite sequences in Turritis glabra
• Detection of the Sec-1 locus of rye by a PCR-based method
• Estimate of outcrossing rates in a rice plant (Oryza sativa L.) under field conditions using a purple grain rice cultivar, Okunomurasaki
• Allocation of a gametocidal chromosome of Aegilops cylindrica to wheat homoeologous group 2
• Molecular mapping of the suppressor gene Igc1 to the gametocidal gene Gc3-C1 in common wheat
• Detection of terminal deletions in barley chromosomes by the PCR-based method
• Dissection of barley chromosome 4H in common wheat by the gametocidal system and cytological mapping of chromosome 4H with EST markers
• Genetic diversity of three Elymus species indigenous to Japan and East Asia (E. tsukushiensis, E. humidus and E. dahuricus) detected by AFLP
• Molecular mapping of the suppressor gene Igc1 to the gametocidal gene Gc3-C1 in common wheat
• Dissection and cytological mapping of barley chromosome 2H in the genetic background of common wheat
• A wheat homologue of PHYTOCLOCK 1 is a candidate gene conferring the early heading phenotype to einkorn wheat
• A wheat homologue of PHYTOCLOCK 1 is a candidate gene conferring the early heading phenotype to einkorn wheat

もっと見る 閉じる

スポンサーリンク