温度馴化したコイアクトミオシンのATPase活性と熱安定性〔英文〕

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Mg2+-ATPase activity and inactivation rate constant of Ca2+-ATPase were measured for actomyosins including intact and desensitized types from 10°C- and 30°C-acclimated carp. Mg2+-ATPase activities of both intact and desensitized actomyosins from the cold-acclimated carp were about twice those from the warm-acclimated carp counterparts. In addition, the inactivation rate constant of the cold-acclimated preparation was larger than that of the warm-acclimated one, when the same type of actomyosin was compared. Actomyosin reconstituted with the 10°C-acclimated desensitized actomyosin and 30°C-acclimated Ca2+ regulatory proteins consisting of tropomyosin and troponin showed Mg2+-ATPase activity and thermostability similar to those of intact actomyosin from the 10°C-acclimated carp. No acclimation temperature-dependent effect of regulatory proteins on the activity or thermostability of actomyosin was observed when the warm-acclimated desensitized actomyosin and the cold-acclimated regulatory proteins were combined.
日本水産學會の論文