芽胞形成菌におけるジピコリン酸生合成部位に関する研究-2-Sporangium細胞内分画のジピコリン酸生合成能について

概要

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The participation of sporangial cytoplasm in dipicolinic acid biosynthesis was studied. When lysozyme was added to sporulating culture of B. subtilis at various stages, the further synthesis of dipicolinic acid was immediately and almost completely inhibited. It was also found that, when isolated forespores were incubated in culure supernatant obtained from sporulating culture, dipicolinic acid was not synthesized.The changes in the distribution of the diketopimelate oxidation system in cells were studied. The oxidative activity was found in the soluble fraction, and not in the residue fraction during all stages of sporulation. The activity was not detected in mechanical disintegrates of forespores and also in lysozyme lysates of the vegetative cells, and the activity suddenly emerged just before the onset of sporulation. The active substance was supposed to be low molecular organic substance by qualitative analyses. From the above results, it was concluded that the cytoplasm surrounding the developing spore is required for dipicolinic acid synthesis.
日本細菌学会の論文