微生物によるビタミンB_1誘導体の変化(III) Escherichia coliによるDisulfide型ビタミンB_1誘導体のビタミンB_1への復帰(2)

概要

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TPD, when incubated with Escherichia coli, was reduced to thiamine and practically all the reduced thiamine was found in the medium, not in cells. There is no difference between reduction of TPD by spheroplasts and that by intact cells. TPD was shown to be reduced equally by ghosts or cytoplasm. Uptake of TPD by E. coli was greater than that of thiamine. TPD uptaken into cells was detected as phosphorylated thamine and free thiamine but not as TPD. Free thiamine was detected more highly in the case of TPD uptake. TPD uptake was not dependent on energy source and not inhibited by pyrithiamine and deoxythiamine in contrast with thiamine uptake. TPD uptake ceased at about 30 min, but occured again when the cells were suspended into fresh medium containing TPD. Release of intracellular thiamine compounds into medium was not observed. On the other hand, TPD uptake was similar to the mode of thiamine uptake in the low concentration of TPD.
日本ビタミン学会の論文
1971-08-25