芽胞形成菌におけるジピコリン酸生合成部位に関する研究-1-芽胞形成過程におけるジピコリン酸の細胞内分布の変化

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Intracellular distribution of dipicolinic acid (DPA) during sporulation was studied by using a strain of B. subtilis. In the initial stage of sporulation, DPA was detectable only in the soluble fraction and the content rapidly decreased with increase in the content of DPA in the residue fraction. In contrast, no DPA was detectable in the supernatant derived from the cells in the stage of maximal sporulation.When the matured spores were mechanically disrupted for a relatively short time, it was found that DPA could easily be released from the matured spores and the kinetic pattern of the release was not parallel with the release of protein and nucleic acid from the spores. On the distribution of DPA in the disintegrated spores, it was also found that this compound is mostly detected in the residual supernatant after ammonium sulfate fractionation.From these results, it was suggested that DPA may be mainly synthesized outside the forespore and then accumulated inside the spore coat in a free from or at least in a loosely bound form.
日本細菌学会の論文