トリフェニルホスフィン還元前処理2, 4-DNPH 法による自動酸化油脂のカルボニル価の測定

概要

論文の詳細を見る
Carbonyl value (CV) of several edible oils were followed during autoxidation at 46°C by two procedures using 2, 4-dinitrophenylhydrazine, the conventional and triphenyl phosphine (TP) reduction procedures, in order to assess the contribution of hydroperoxides to the CV determination of oxidized oils.<BR>CV determined by the conventional procedure (Conv-CV) was always higher than that by the TP-reduction procedure (TP-CV). The discrepancy between these methods increased linearly with peroxide value. However, the ratio of Conv-CV/TP-CV reached a maximum, of around 3 to 4, at the end of the induction period. The time courses of Conv-CV in the initial stage of oxidation were basically the same as those of POV, because most carbonyls assayed by this method were produced by the decomposition of hydroperoxides.<BR>Therefore, a procedure, not affected by peroxides, should be established for CV measurement of autoxidized oils even in the early stage of autoxidation. The TP procedure is simple, and TP and TP oxides have no effect on CV determination. TP-CV was found closely correlated to the sensory evaluation of the odor and flavor of oxidized oils. The TP procedure was confirmed very useful for CV measurement of autoxidized oils.
社団法人日本油化学会の論文