水棲生物におけるP32の吸収および代謝に関する研究-2-

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Several studies were reported on the separation of the acid-soluble nucleotides in fish tissues2-5) and an earthworm, Lumbricus terrestris6), by the ion-exchange chromatography. However, no works have been made, in the aquatic organism, of the fractionation of directly uptaken phosphorus from the medium into various phosphorus compounds. The present study deals with the incorporation of absorbed P32 into various acid-soluble nucleotides when a sludge worm, Rhizodrilus limasus, is exposed to P32 labeled water. The acid-soluble phosphorus compounds which were extracted from the P32 labeled sludge worm with perchloric acid were fractionated into 13 fractions by a column chromatography according to the COHN and CARTER method8) (Table 1 and Fig. 1). Inorganic phosphate, AMP, ADP and ATP which contained P32 were identified, based on the ultraviolet absorption curves (Fig. 2) and elution positions observed when the extract was added with H3P32O4 and respective authentic nucleotides. Thirty-six per cent and thirty-five per cent of P32 which existed in the extract were found in the inorganic phosphate and the adenosine polyphosphate fractions, respectively (Table 2). Excellent recoveries were obtained in this ion-exchange chromatography (Table 3).
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