魚類におけるクロロフェノール類の代謝に関する研究-VIII : キンギョ胆汁中のフェニル-β-グルクロナイドの単離・同定

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The isolation, identification and accumulation of a conjugated-phenol in bile of goldfish, Carassius auratus, exposed to phenol have been studied. The accumulation of the conjugated-phenol in the bile of goldfish increased rapidly with exposure time, whereas the concentration of free-phenol remained at a much lower level. The conjugated-phenol was isolated by treating the bile on activated charcoal columns eluting with acetone-water, and finally passing the concentrated eluate through a Sephadex G-10 column. The isolated conjugate was identified as phenyl-β-glucuronide by hydrolysis with hot acidic solution and with β-glucuronidase to release free-phenol, by thin-layer and gas-liquid chromatography, and by the molar ratio of phenol to glucuronic acid. However, phenylsulfate which is another conjugate excreted by goldfish into surrounding water was not detected in the bile.
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