魚類におけるクロロフェノール類の代謝に関する研究-V : キンギョ生成フェノール抱合体の単離と同定

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A conjugated phenol excreted by goldfish, Carassius auratus, into the culture medium was isolated by treating the medium with activated charcoal columns, followed by elution with acetone-water and/or ammonia mixtures, and finally passing the concentrated eluate, under reduced pressure, through a Sephadex G-10 column. The isolated conjugate was identified as phenylsulfate by elution patterns on columns of charcoal and Sephadex, pre-cipitation with BaCl2, hydrolysis on heating in acidic solution to release free-phenol, thin layer chromatography, UV-absorption spectra and the molar ratio of phenol to SO4, com-paring it with the synthesized phenylsulfate. Glucuronide was not detected, although ADAMSON et al. (1967, 1972, 1974) have reported the formation of glucuronides for some phenolic compounds in fishes. The present study shows that sulfate conjugation is one of the most general detoxication mechanisms for phenolic compounds in fish, at least in goldfish, contrary to the conclusion of MAICKEL et al. (1958) that goldfish were completely incapable of forming either glucuronides or sulfates for phenols.
公益社団法人日本水産学会の論文