Production of Transgenic Bovine Cloned Embryos Using Piggybac Transpositioin

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概要

• 論文の詳細を見る

• Transgenic research on cattle embryos has been developed to date using viral or plasmid DNA delivery systems. In this study, a different gene delivery system, piggybac transposition, was employed to investigate if it can be applied for producing transgenic cattle embryos. Green or red fluorescent proteins (GFP or RFP) were transfected into donor fibroblasts, and then transfected donor cells were reprogrammed in enucleated oocytes through SCNT and developed into pre-implantation stage embryos. GFP was expressed in donor cells and in cloned embryos without any mosaicism. Induction of RFP expression was regulated by doxycycline treatment in donor fibroblasts and pre-implantational stage embryos. In conclusion, this study demonstrated that piggybac transposition could be a mean to deliver genes into bovine somatic cells or embryos for transgenic research.

著者

• LEE Byeongchun

  Department of Theriogenology & Biotechnology, College of Veterinary Medicine

• Han Hyo

  Department Mathematics Pusan National University

• Lee Won

  Embryo Research Center Seoul Dairy Corporation

• Kim Bong

  Department Of Anatomy College Of Medicine Pusan National University

• Jang Goo

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Kim Su

  Department Of Agricultural Chemistry And Research Center For New Bio-materials In Agriculture Seoul

• Lee Byeong

  Department Of Information And Communications Gwangju Institute Of Science And Technology

• Choi Woo

  Department Of Dermatology Sanggye Paik Hospital Inje University College Of Medicine

• KIM SuJin

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institut

• SAADELDIN Islam

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institut

• CHOI WooJae

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institut

• LEE SongJeon

  Embryo Research Center in Seoul Milk Coop.

• LEE WonWu

  Embryo Research Center in Seoul Milk Coop.

• KIM BongHan

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institut

• HAN HyoJun

  Department of Chemistry, YonSei University

• BANG DuHee

  Department of Chemistry, YonSei University

• LEE Song

  Embryo Research Center in Seoul Milk Coop.

• BANG Du

  Department of Chemistry, YonSei University

• SAADELDIN Islam

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute, Seoul National University

• HAN Hyo

  Department of Chemistry, YonSei University

• LEE Byeong

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute, Seoul National University

• CHOI WOO

  Department of Chemical Engineering, Doshisha University

• KIM Bong

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine and Research Institute, Seoul National University

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