In vivoならびにIn vitroで成熟させた異なる生殖周期に採取した犬の卵母細胞中のグルタチオン濃度(臨床繁殖学)

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• 卵母細脳中のグルタチオン濃度は細胞質成熟のマーカーとして重要である.そこで,in vivoとin vitroで成熟させた犬卵母細胞中のグルタチオン濃度について比較検討した.In vivoで成熟させた卵母細胞は排卵72時間後に開腹し,卵管を環流して採取した.異なる性周期の卵巣を採取して得られた卵母細胞は,その直径が120μm未満と120μm以上の2群にわけ,それぞれ10%FBS,2.2mg/ml NaHCO_3, 2.0μs/ml estrogen, 0.5μ2/ml FSH,0.03IU/ml hCG,1%penicillin-streptomycinを添加したTCM-199培養液で,50μMβ-mercantoethanol添加あるいは無添加で72時間培養した.GSH濃度はDTNB-GSSG reductase recycling法で測定した.未成熟な犬卵母細腹中のGSH濃度は発情休止期,卵胞期,黄体期,それぞれの120μm未満と120μm以上の卵母細胞で,2.9と3.8,3.5と6.8,3.1と6.5pM/卵母細胞と有意に120μm以上の卵母細胞が高かった(p<0.05).In vivoで成然した卵母細腹中のGSH濃度(19.2pM/卵母細胞)はin vitroで成熟させた卵母細胞(β-mercaotoethanol無添加4.1-8.1pM/卵母細胞,添加5.7-13.2pM/卵母細胞)よりも有意な高値(p<0.05)であった.したがってβ-mercantoethanol添加はin vitroで培養した卵母細胞のGSH合成を増加させ,また卵胞期ならびに黄体期に採取した卵母細胞は発情休止期に採取した卵母細胞よりもin vitroの成熟に優れていると考えられた.

• 社団法人日本獣医学会の論文
• 2007-06-25

著者

• KIM Min

  Department of Internal Medicine, Chonnam National University Hospital

• PARK Jung

  Department of East-West Medicine, Graduate School, Kyung Hee University

• OH Hyun

  Department of Electrophysics, Kwangwoon University

• LEE Byeong

  Department of Information and Communications, Gwangju Institute of Science and Technology (GIST)

• Kim Min

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• KANG SUNG

  Department of Urology, College of Medicine, The Catholic University of Korea

• Hong So

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive

• JANG Goo

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive

• Oh Hyun

  Department Of Electrophysics Kwangwoon University

• Kang Sung

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• HOSSEIN Mohammad

  Department o fTheriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive

• FIBRIANTO Hera

  Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive

• KIM Hye

  Haemaru Small Animal Referral Hospital

• Lee Byeong

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Hossein Mohammad

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Hossein Mohammad

  Department O Ftheriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Oh Hyun

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Hong So

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Jang Goo

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Lee Byeong

  Department Of Information And Communications Gwangju Institute Of Science And Technology

• Park Jung

  Department Of Chemistry Gyeongsang National University

• Park Jung

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Fibrianto Hera

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Kim Min

  Department Of Chemical Engineering Cheju National University

• Kang Sung

  Department Of Ophthalmology University Of Ulsan College Of Medicine Asan Medical Center

• Lee Byeong

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Kang Sung

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Kang Sung

  Department Of Biological Science Kongju National University

• Park Jung

  Department Electrical Engineering Busan National University

• Kim Min

  Department of Applied Science, Korea Maritime University, Busan 606-791, Korea

• KIM Min

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 305–764, Korea

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