Effect of Potassium Simplex Optimization Medium and NCSU23 Supplemented with Beta-mercaptoethanol and Amino Acids of In Vitro Fertilized Porcine Embryos
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概要
- 論文の詳細を見る
The present study was conducted to examine the comparative efficacy of potassium simplex optimization medium (KSOM) and North Carolina State University (NCSU)-23 medium supplemented with beta-mercaptoethanol (β-ME) and amino acids (AA) on the developmental competence of porcine in vitro fertilized (IVF) embryos. Four experiments were conducted. KSOM and NCSU-23 medium were used to culture porcine parthenogenetic (Exp. 1) and IVF (Exp. 2) embryos. KSOM and NCSU-23 were equally effective in supporting porcine parthenogenetic and IVF embryo development from the 1-cell stage to blastocysts. The NCSU-23 medium (Exp. 3) and KSOM (Exp. 4) were supplemented with amino acid (AA; 5 μl/ml non-essential amino acids + 10 μl/ml essential amino acids) and/or 10 μM beta-mercaptoethanol (β-ME). The quality of blastocysts from Exp. 3 and 4 was evaluated by counting the number of total cells and determining the ratio of the inner cell mass (ICM) to trophoectoderm (TE) cells. Supplementing with AA and β-ME or βME alone in NCSU-23 produced significant (p<0.05) differences in terms of rate of cleavage to the 2- to 4- cell (80.8 to 85.4% vs. 73.6%) and blastocyst (30.4 to 30.5 vs. 23.5%) stages and the number of TE (51.4 to 53.8 vs. 35.8) and total cells (67.2 to 71.2 to 48.8) over the control group. On the other hand, supplementing KSOM with AA and/or β-ME produced significant (p<0.05) differences in terms of rate of cleavage to the 2- to 4-cell (78.8% vs. 67.7%) and morula (57.8% vs. 46.3%) stages and the number of ICM (18.6 to 19.2 vs. 11.6) and total cells (62.8 to 70.6 vs. 42.8) over control group. In conclusion, our study demonstrates that both KSOM and NCSU-23 medium supplemented with AA and β-ME and/or only βME alone are superior to normal KSOM and NCSU-23 for porcine IVF embryo culture in terms of embryo developmental competence and quality.
- 日本繁殖生物学会の論文
- 2006-10-01
著者
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Kim Ji-hye
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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LEE Byeong
Department of Information and Communications, Gwangju Institute of Science and Technology (GIST)
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Woo Jeong
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Woo Jeong
Department Of Endocrinology And Metabolism Kyung Hee University School Of Medicine:research Institut
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KANG SUNG
Department of Urology, College of Medicine, The Catholic University of Korea
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Kang Sung
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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HOSSEIN Mohammad
Department o fTheriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive
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HASHEM Md.
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive
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KIM Sue
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive
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LEE So-Hyun
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive
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KOO Ok-Jae
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive
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PARK Seon
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive
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LEE Eu
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National Unive
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Lee Byeong
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Hossein Mohammad
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Hossein Mohammad
Department O Ftheriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Lee Eu
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Lee So-hyun
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Kim Sue
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Koo Ok-jae
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Park Seon
Department Of Obstetrics And Gynecology College Of Medicine Korea University
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Hashem Md.
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Lee Byeong
Department Of Information And Communications Gwangju Institute Of Science And Technology
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Kang Sung
Department Of Ophthalmology University Of Ulsan College Of Medicine Asan Medical Center
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Lee Byeong
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Kang Sung
Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers
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Kang Sung
Department Of Biological Science Kongju National University
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Lee So-hyun
Department Of Biological Science College Of Natural Sciences Institute Of Biotechnology Wonkwang Uni
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Hashem Md.
Department Of Chemistry Kumamoto University
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