Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using Somatic Cell Nuclear Transfer

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概要

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• A recent emerging technology, somatic cell nuclear transfer (SCNT), has been considered for conserving threatened or endangered species. Sapsaree is a native breed in Korea and has been designated as a Natural Monument. The aim of this study was to produce a Sapsaree by SCNT for breed conservation. Donor fibroblasts from a 9-year-old male Sapsaree were placed into the perivitelline spaces of enucleated in vivo matured oocytes and fused electrically. A total of 309 cloned embryos were transferred into the oviducts of 15 naturally synchronized recipients. Two recipients were diagnosed as pregnant, and each delivered one cloned puppy, both of which weighed 530 g. Overall, this study demonstrated that an endangered canine breed can be conserved by SCNT.

著者

• LEE Byeongchun

  Department of Theriogenology & Biotechnology, College of Veterinary Medicine

• Jang Goo

  Department Of Theriogenology And Biotechnology College Of Veterinary Medicine Seoul National Univers

• Park Chankyu

  Department Of Animal Biotechnology College Of Animal Bioscience And Technology Konkuk University

• Kim Minkyu

  Department Of Biomedical Engineering School Of Medicine Kyungpook National University

• Oh Hyunju

  Department Of Rehabilitayion Science Graduate School Daegu University

• KIM MinKyu

  Department of Theriogenology & Biotechnology, Seoul National University

• HONG SoGun

  Department of Theriogenology & Biotechnology, Seoul National University

• KANG JungTaek

  Department of Theriogenology & Biotechnology, Seoul National University

• PARK JungEun

  Department of Theriogenology & Biotechnology, Seoul National University

• HA JiHong

  School of Life Science and Biotechnology, Kyungpook National University

• KIM DaeYong

  Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University

• KANG JungTaek

  Department of Theriogenology & Biotechnology, Seoul National University

• HONG SoGun

  Department of Theriogenology & Biotechnology, Seoul National University

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