The in vitro primary response of peripheral blood mononuclear cells to sheep red blood cells during human growth and development and the effect of interleukin 1 and 2 on the antigen-specific immunogulobulin production.

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Human cord blood lymphocytes respond well to concanavalin A or phytohemagglutinin to proliferate, and human cord blood B cells produce immunoglobulins, especially IgM, after the stimulation by Epstein-Barr virus which is known to be polyclonal B cell activator. Pokeweed mitogen (PWM) which is a T cell-dependent polyclonal B cell activator poorly induces immunoglobulin from cord blood lymphocytes, while adult peripheral blood mononuclear cells (PBMC) well produce polyclonal immunoglobulins by PWM stimulation. However, antigen-specific IgM antibody is produced in the intrauterine infection by foetus. Hence, we tried to examine whether cord blood lymphocytes can respond to sheep red blood cells (SRBC) to produce SRBC-specific antibody utilizing the in vitro culture system consisting of cord blood lymphocytes and SRBC as antigen. In this culture system, SRBC-specific immunoglobulin secreting cells (IgSC) are enumerated after the 8 day culture, and SRBC-specific immunoglobulins secreted by B cells are considered to be IgM. In our experiments, cord blood lymphocytes produced no IgSC specific for SRBC, while adult PBMC produced 310_??_3.87 (geometric mean _??_ SD) SRBC-specific IgSC/107 cells. PBMC derived from 1 to 15-year-old children responded to SRBC in vitro to produce 24_??_3.75 SRBC-specific IgSC/107 cells intermediately. SRBC-specific IgSC were apparently present in the peripheral blood in childhood compared to cord blood, but the number of IgSC in childhood was significantly lower than that in adult (p<0.001). It was reported in the accompanying paper that interleukin 2 (IL 2) augmented SRBC-specific IgM antibody production and the number of SRBC-specific IgSC when exogenous IL 2 was added to the culture consisting of adult PBMC and SRBC as antigen. It was also clarified in this paper that human recombinant interleukin 1 (IL 1) augmented the number of SRBC-specific IgSC in this assay system. Hence, exogenous IL 1 or IL 2 was added to the in vitro culture consisting of cord blood lymphocytes or PBMC obtained from 1 to 15-year-old children or adult individuals with SRBC as antigen. Cord blood lymphocytes produced no SRBC-specific IgSC in the presence of exogenous IL 1 or IL 2, while PBMC in childhood remarkably responded to SRBC in the presence of IL 1, IL 2, and produced large number of SRBC-specific IgSC which were almost equal to the number of SRBC-specific IgSC of adult PBMC cultured with SRBC in vitro in the absence of exogenous IL 1 or IL 2. Childhood PBMC were essentially similar to adult PBMC in the response to SRBC in the presence of IL 1 or IL 2, while childhood PBMC less effectively produced SRBC-specific IgSC in the absence of IL 1 or IL 2 than adult PBMC.
日本臨床免疫学会の論文

The in vitro primary response of peripheral blood mononuclear cells to sheep red blood cells during human growth and development and the effect of interleukin 1 and 2 on the antigen-specific immunogulobulin production.

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