The effect of recombinant interleukin 2 on the in vitro primary response of human peripheral blood mononuclear cells to sheep red blood cells.

概要

論文の詳細を見る
The effect of interleukin 2 (IL 2) on the primary antigen-specific immunoglobulin production of human peripheral blood mononuclear cells (PBMC) has been unknown yet. It has already been reported that human PBMC respond to sheep red blood cells (SRBC) in vitro and produce SRBC-specific immunoglobulin secreting cells (IgSC). Hence, it seems interesting to study how human recombinant IL 2 works in SRBC-specific immunoglobulin production of human PBMC.Utilizing this in vitro system, we showed that human recombinant IL 2 increased antigen-specific IgSC at the moderate concentration (100 u/ml). However, it did not increase antigen-specific IgSC at the higher concentration (1, 000 u/ml). SRBC-specific IgM antibody produced in the culture was also assessed utilizing the enzyme-linked immunosorbent assay, and it was also shown that the moderate concentration of IL 2 (100 u/ml) augumented antigen-specific IgM antibody production, but the higher concentration of IL 2 (1, 000 u/ml) did not. The reason why IL 2 at the high concentration did not augment the immunoglobulin production was discussed in this paper.Anti-Tac is a well-known IL 2 receptor antibody. When anti-Tac was added to the culture of PBMC and SRBC, anti-Tac markedly inhibited the expansion of IgSC (20μg/ml), and it was shown that endogenous IL 2 was required for the induction of SRBC-specific IgSC. When recombinant IL 2 was added to the cultures in the co-presence of anti-Tac (20μg/ml), immunoglobulin production and expansion of IgSC were restored at the high concentration of exogenous IL 2 (1, 000 u/ml). In the further experiment, IL 2 was added to the culture of PBMC and SRBC on day 0, day 3, or day 6, while anti-Tac was added to the other culture on day 0, day 2, day 4, or day 6. IL 2 augmented the number of IgSC when added to the culture by day 3, and anti-Tac inhibited the expansion of IgSC when added to the culture by day 4.In this report, we demonstrated that the interaction between IL 2 receptor and IL 2 generated endogenously was required in the antigen-specific immunoglobulin production. The addition of exogenous IL 2 at the moderate concentration to the culture remarkably augmented the antigen-specific immunoglobulin production and increased the number of antigen-specific IgSC. The presence of IL 2 on day 3 and 4 in this 8-day culture was essential for the expansion of antigen-specific IgSC.
日本臨床免疫学会の論文

The effect of recombinant interleukin 2 on the in vitro primary response of human peripheral blood mononuclear cells to sheep red blood cells.

スポンサーリンク

概要

著者

関連論文

スポンサーリンク