EFFECTS OF VARIOUS COMPOUNDS ON LIPID PEROXIDATION MEDIATED BY DETERGENT-SOLUBILIZED RAT LIVER NADPH-CYTOCHROME <I>C</I> REDUCTASE
スポンサーリンク
概要
- 論文の詳細を見る
A reconstituted lipid peroxidation system containing NADPH-cytochrome <I>c</I> reductase isolated from detergent-solubilized rat liver microsomes was used to determine the effects of several compounds, including drugs, on the lipid peroxidation activity. EDTA and ferrous ion were essential requirements for reconstitution of the activity. The addition of 1, 10-phenanthroline to the system containing both EDTA and ferrous ion further enhanced the activity. Pyrocatecol, thymol, <I>p</I>-aminophenol, imipramine, <I>p</I>-chloromercuribenzoate (PCMB) and α-tocopherol exhibited strong inhibition, aniline, N-monomethylaniline, aminopyrine, benzphetamine, SKF 525-A and NADP exhibited moderate inhibition, and phenol, benzoic acid, acetanilide and nicotinamide exhibited less or no inhibition at the concentrations lower than 1000 μM. Metal ions such as Hg<SUP>+</SUP>, Hg<SUP>2+</SUP>, Co<SUP>2+</SUP>, Cu<SUP>2+</SUP>, Mn<SUP>2+</SUP> and U<SUP>6+</SUP> inhibited lipid peroxidation strongly. In addition, Cd<SUP>2+</SUP>, St<SUP>2+</SUP> and Ca<SUP>2+</SUP> exhibited less potent to moderate inhibition, and Ba<SUP>2+</SUP> and Mg<SUP>2+</SUP> were without effects on the activity. Among sulfhydryl compounds tested, dithiothreitol inhibited lipid peroxidation to a greater extent than did the other three compounds, glutathione, cysteine and mercaptoethanol.
- 社団法人 日本薬理学会の論文
著者
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Kamataki Tetsuya
Department Of Biochemical Pharmacology Faculty Of Pharmaceutical Sciences Chiba University
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Sugita Osamu
Department Of Urology Kochi Medical School
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Kitagawa Haruo
Department Of Biochemical Pharmacology Faculty Of Pharmaceutical Sciences Chiba University
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Naminohira Shigeru
Department Of Toxicology School Of Pharmacy Hokuriku University
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NAMINOHIRA Shigeru
Department of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, Chiba University
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KAMATAKI Tetsuya
Department of Pharmacology, Keio University School of Medicine
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SUGITA Osamu
Department of Biochemical Pharmacology, Faculty of Pharmaceutical Sciences, Chiba University
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KITAGAWA Haruo
Department of Biochemical Pharmacology and Biotoxicology, Faculty of Pharmaceutical Sciences, Chiba University
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KAMATAKI Tetsuya
Department of Analytical Bio chemistry, School of Pharmacy, Hokkaido University
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