EFFECTS OF LYOPHILIZATION AND STORAGE OF RAT LIVER MICROSOMES ON ACTIVITY OF ANILINE HYDROXYLASE, CONTENTS OF CYTOCHROME b<SUB>5</SUB> AND CYTOCHROME P-450 AND ANILINE-INDUCED P-450 DIFFERENCE SPECTRUM
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概要
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Effects of lyophilization and storage of rat liver 9, 000×g supernatant fraction or microsomes on activity of aniline hydroxylase, contents of cyt. P-450 and cyt. b<SUB>5</SUB> and aniline-induced P-450 difference spectrum were studied. The lyophilization of rat liver 9, 000×g supernatant fraction or microsomes produced about 20% decrease in the activity of aniline hydroxylase. Aniline hydroxylase was stably stored for 6 months if lyophilized 9, 000×g supernatant fraction of rat liver had been stored in an atmosphere of nitrogen or in a low pressure of air. Activity of aniline hydroxylase and contents of cyt. P-450 and cyt. b<SUB>5</SUB> were not reduced during aerobical storage for a week. When lyophilized microsomes were stored for 6 months anaerobically, aniline hydroxylase, cyt. P-450 and cyt. b<SUB>5</SUB> were more stable as compared to aerobical storage. However, activity of aniline hydroxylase was reduced approx. 40% by the storage. The reduction of aniline hydroxylase activity was more significant than that of contents of cyt. P-450 and cyt. b<SUB>5</SUB>. Reduction of either activity of aniline hydroxylase or contents of cyt. P-450 and cyt. b<SUB>5</SUB> was more marked when lyophilized microsomes were stored with glycerol. Aniline hydroxylase in lyophilized 9, 000×g supernatant fraction from phenobarbital treated rabbit was stably stored, and aerobically little activity was lost during storage for 7 months.
- 社団法人 日本薬理学会の論文
著者
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Kamataki Tetsuya
Department Of Biochemical Pharmacology Faculty Of Pharmaceutical Sciences Chiba University
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Kitagawa Haruo
Department Of Biochemical Pharmacology Faculty Of Pharmaceutical Sciences Chiba University
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KITAGAWA Haruo
Department of Biochemical Pharmacology and Biotoxicology, Faculty of Pharmaceutical Sciences, Chiba University
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KAMATAKI Tetsuya
Department of Analytical Bio chemistry, School of Pharmacy, Hokkaido University
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