無細胞反芻胃液の蛋白質分解活性に及ぼす繊毛虫類の影響

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It is already shown that the cell free rumen liquid has proteolytic activity, and its activity is very unstable. This experiment was carried out in order to demonstrate the occurrence of extracellular proteolytic enzymes of rumen ciliate protozoa and their activities in the rumen liquid. Two goats(2 years old, female, body wt. 50 kg and 3 years old, castrated male, body wt. 70 kg) and one cow(2 years old, body wt. 500 kg) fitted with rumen fistulas were used. These goats were fed on alfalfa hay and mixed concentrate feed according to the usual method, and the cow was fed on the daily rations consisting 3 kg of rice straw and 4 kg of mixed concentrate feed. In one goat the protozoa-free stage was worked out after the conventional stage. Defaunation was carried out by method of ABOU AKKADA et al.(1968). Rumen liquid was obtained from rumen contents taken through rumen fistula before and after feeding from which cell free rumen liquid was prepared by centrifugation. Results obtained are summarized as follows: 1). Large amount of casein was decomposed by cell free rumen liquid at the conventional stage than at the protozoa free stage, and such tendency was remarkable after feeding. 2). Amount of casein decomposed by cell free rumen liquid was markedly increased by reduction with dithiothreitol and vise versa by oxidation with o-iodosobenzoic acids). The proteolytic activity of cell free rumen liquid taken from goat and cow were the largest about 1 hr after feeding. 4). Casein, protein powder from soybean and wheat gluten were decomposed more easily in order by cell free rumen liquid, while zein was not decomposed. From the facts above-mentioned, it is demonstrated that the rumen ciliate protozoa excrete proteolytic enzymes into the rumen liquid and the proteolytic activity in the cell free rumen liquid is promoted remarkably in a short time after feeding.
社団法人日本畜産学会の論文

無細胞反芻胃液の蛋白質分解活性に及ぼす繊毛虫類の影響

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