Mechanisms of NK cell activation stimulated by CD2 : granzyme B is released by CD2 crosslinking-stimulation on NK92 cells
スポンサーリンク
概要
- 論文の詳細を見る
Natural killer (NK) cells play a key role in the innate immune system through the rapid secretion of cytokines and the ability to lyse virally infected cells or tumor cells. CD2 are surface glycoprotein receptors and important for NK cell activation. Nevertheless, activation of NK92 cells through CD2 crosslinking-stimulation has not been completely understood. Here we analyzed the effects of CD2 crosslinking-stimulation on NK92 cell activation. We confirmed the expression of CD2 on the NK92 cell surface. The majority of NK92 cells expressed CD2 on their cell surfaces. We found that CD2 crosslinking-stimulation enhanced release of granzyme B from NK92 cells. CD2 crosslinking-stimulation markedly induced phosphorylation of extracellular signal-regulated kinase (ERK)1/2 compared with nonstimulated NK92 cells. Furthermore, we discovered that tyrosine phosphorylation of ERK1/2 was quickly induced within 1 min in an antibody concentration dependent manner. Mitogen-activated protein kinase extracellular signal-regulated kinase (MEK) inhibitor U0126 is a chemically synthesized organic compound that inhibits activation of ERK1/2. U0126 treatment inhibited the release of granzyme B, compared with CD2 crosslinking-stimulated NK92 cells, in a concentration-dependent manner. These results suggest that CD2 crosslinking-stimulation enhances the release of granzyme B and phosphorylation of ERK1/2, which are critical events during NK cell-mediated cytotoxicity.
- 2012-10-00
著者
-
Nishikawa Yasuo
Department of Physiology, Osaka Dental University
-
Uchihashi Kenji
Department Of Physiology Osaka Dental University
-
OHSHIMA Takashi
Department of Agricultural Chemistry, Utsunomiya University
-
Fujimoto Tetsuya
Department Of Chemistry Faculty Of Science Yamagata University
-
Suwabe Takeshi
Department Of Physiology Osaka Dental University
-
Inoue Hiroshi
Departmcnt Of Biology Faculty Of Science Kyushu University:(present)department Of Biology Kyushu Den
-
Meng Shu
State Key Laboratory of Oral Diseases, Sichuan University:Department of Periodontology, West China College of Stomatology, Sichuan University
-
Nishikawa Yasuo
Department of Electronic Engineering, Faculty of Engineering, Tokyo Institute of Polytechnics,
-
Zhao Lei
State Key Laboratory of Oral Diseases, Sichuan University:Department of Periodontology, West China College of Stomatology, Sichuan University
-
Wu Ya-fei
State Key Laboratory of Oral Diseases, Sichuan University:Department of Periodontology, West China College of Stomatology, Sichuan University
-
Zhang Lin
State Key Laboratory of Oral Diseases, Sichuan University
-
Inoue Hiroshi
Department of Physiology, Osaka Dental University
-
Fang Yi-ru
Dental Oriental Medicine, Osaka Dental University
-
Hirano Shun-ichiro
Department of Physiology, Osaka Dental University
-
Aachi Tetsuya
Department of Physiology, Osaka Dental University
-
Suwabe Takeshi
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
-
Uchihashi Kenji
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
-
Hirano Shun-ichiro
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
-
ZHANG Lin
State Key Laboratory for Advanced Metals and Materials, Beijing Key Laboratory for Powder Metallurgy and Particulate Materials, University of Science and Technology Beijing
-
Inoue Hiroshi
Deparment of Neurosurgery, Gunma University School of Medicine and Gamma Unit Center, Hidaka Hospital
-
MENG Shu
State Key Laboratory of Oral Diseases, Sichuan University
-
ZHAO Lei
State Key Laboratory of Oral Diseases, Sichuan University
関連論文
- Projection of lingual muscle pain to the brain
- Invasive Ductal Carcinoma of the Pancreas, Measuring 2cm or Less in Greatest Dimension : A Clinicopathologic Study with an Analysis of Aberrant Chromosomal Regions Using CGH
- Sex-Related Differences in the Risk Factor Profile and Medications of Patients With Atrial Fibrillation Recruited in J-TRACE
- A Case of Japanese Encephalitis:CT and MRI Findings in Acute and Convalescent Stages
- An Equivalent Circuit Model for Predicting EM Radiation from Stripline Structure with a Thin Wire
- An Equivalent Circuit Model for Predicting EM Radiation from Stripline Structure with a Thin Wire
- Involvement of Carboxyl Groups of the PSII Reaction Center Proteins in Photoactivation of the Apo-Water-Oxidizing Complex
- Changing size of the bronchogenic cyst, A case report
- Effects of premedication on salivary secretion in the rat submandibular gland
- Diagnosis and Treatment of Acute Cardiac Tamponade by the Subxiphoid Approach
- Reconstruction of Sternal Defects with Autologous Bone Grafts and Myocutaneous Flap of the Latissimus Dorsi Muscle
- P578 RESPONSES OF THE LEFT VENTRICLE WITH SYMPATHETIC NEURAL DYSFUNCTION TO ADRENERGIC STIMULATION BY EXERCISE
- Expression of Xeroderma Pigmentosum Group G (XPG) in Sporadic Breast Carcinoma
- Lead tolerance and accumulation in the gametophytes of the fern Athyrium yokoscense
- Role of Hepatic STAT3 in the Regulation of Lipid Metabolism
- On the Neutrino Theory of Light
- B-22 Morphological Expression and Localization of Interleukin-6 (IL-6) in Pituitary Adenomas
- Guideline for Denture Relining
- Y-931, a Novel Atypical Antipsychotic Drug, Is Less Sensitive to Oxidative Phenomena
- Effect on mastication of experimental occlusal interferences in rats
- Cerebellar Abscess Associated with Pulmonary Arteriovenous Fistula and Hereditary Hemorrhagic Telangiectasia : Case Report
- Prediction of EM Radiation from a PCB Driven by a Connected Feed Cable
- Functional maxillary denture model for rats : Investigations based on masticatory performance and masseter electromyography
- Extracellular Glutamate Release in the Edentulous Rat Hippocampus following Tetanic Stimulation : in vivo Study by Microdialysis
- Activity changes in the open-field test related to loss of rat molar occlusal support
- Effect of Height of Occlusal Interference on Dopamine Release in the Prefrontal Cortex of Rats
- Formation of Singlet Oxygen-Like Active Oxygen Species in System Containing Nickel(II) Complex and Aliphatic Aldehyde
- Different Coronary Blood Flow Increase in Left Ventricular Hypertrophy Due to Hypertension Compared to Hypertrophic Cardiomyopathy at Elevated Heart Rate
- Multiple hepatic pseudotumors on sonography
- Percutaneous Transhepatic Cholangioscopic Lithotomy of a Common Bile Duct Stone through the Gallbladder Route
- Letters to the editor
- CARDIOVASCULAR TOLERANCE IN UNSEDATED UPPER GASTROINTESTINAL ENDOSCOPY : PROSPECTIVE RANDOMIZED COMPARISON BETWEEN TRANSNASAL AND CONVENTIONAL ORAL PROCEDURES
- Generation of Monopole Ultrasonic Pulse from a Circular Source and It's On-axis Pressure Characteristics
- Experiments on Monopole Ultrasonic Pulse Radiated from Piezoelectric Polymer Film Transducers
- Mixed Pituitary Adenoma and Gangliocytoma Associated with Acromegaly : Case Report
- Effective Bromocriptine Theraph for Large,Calcified Prolactinoma : -Pathogenesis of Calcifications-
- -219-TREATMENT OF ACUTE LEFT-SIDED HEART FAILURE WITH INTRAMUSCULAR INJECTION OF CHLORPROMAZINE : Cardiac FAILURE : PROCEEDINGS OF THE 42nd ANNUAL MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Immune Response of Regional Lymph Nodes in Patients with Lung Cancer
- Measurement Method of Ultrasonic Velocity in Liquid and Solid Using Continuous Wave Signal
- Measurement of Micrometer Order Displacement Using Phase-Shift Type Ultrasonic Correlation System in MHz Range
- Measurement of Ultrasonic Attenuation in Magnetic Fluid and its Application to Surface Profiling
- Simultaneous analysis of human plasma catecholamines by high-performance liquid chromatography with a reversed-phase triacontylsilyl silica column
- Thalamic nociceptive neurons of the cat responsive to masseter muscle afferent input
- Effect of conditioning stimulation of the fornix on the response of thalamic nociceptive neurons to tooth pulp stimulation
- Functional properties of nociceptive neurons in the nucleus centralis lateralis of the cat thalamus
- Rat small hepatocytes for liver support : growth and metabolic activities in culture
- Maintenance of morphology and function of mixed liver cell spheroids under collagen gel environment
- A Case of Intra-pulmonary Seminoma
- A Study on the Conditions for Generation of Monopole Ultrasbnic Pulse by Piezoelectric Polymer Film Transducers
- Development of a Detector of Variation of Underground Water Level Using Ultrasound : Acoustical Measurements and Instrumentation
- PJ-012 Electrophysiological and Anatomical Differences in Retrograde Slow Pathway between Fast-slow and Slow-slow Form of Atypical Atrioventricular Nodal Reentry(PJ002,Atrial/Supraventricular Arrhythmia (Clinical/Pathophysiology) (A),Poster Session (Japan
- Photocatalytic Activities for Carbon Dioxide Reduction of TiO_2 Microcrystals Prepared in SiO_2 Matrices Using a Sol-Gel Method
- Measurement of Acoustic Properties of Magnetic Fluid and Its Application to Nondestructive Evaluation
- Regional Expression of CXCL12/CXCR4 in Liver and Hepatocellular Carcinoma and Cell-cycle Variation during in vitro Differentiation
- Video-Assisted Thoracoscopic Surgery under local anesthesia for right empyema secondary to aspiration pneumonia caused by esophageal achalasia : case report
- Is it permissible to omit mediastinal dissection for peripheral non-small-cell lung cancers with tumor diameters less than 1.5 cm?
- Regeneration of Hemisectioned Spinal Cords With and Without Supporting Materials
- A Mouse model of a sudden death Induced by Noise Exposure is Useful to Investigate Human Responses to Physical Stress
- Changes in retention of electroformed telescope crowns over long-term use
- Retentive force characteristics of electroformed telescope crowns related to protection of abutment teeth
- Retentive force characteristics of electroformed telescope crowns
- Ultrastructural Investigation of Nerve Regeneration in Vivo : Comparison with in Vitro Neurite Formation
- Neurotoxicity of perfluorooctane sulfonate (PFOS) in rats and mice after single oral exposure
- L-myc Restriction Fragment Length Polymorphism in Japanese Patients with Esophageal Cancer
- Laser - Induced Photoionization of Two - Electron - Reduced Viologen
- Expression of Cyclooxygenase-1, -2, Peroxisome Proliferator-activated Receptor gamma in Human Colorectal Carcinoma.
- Effect of hydrogen peroxide on the fluorescence and the C550 absorption change of spinach chloroplast fragments
- Oxidation-reduction reactions between electron transfer components and hydrogen peroxide in photosystem II of chloroplasts
- リンゴ酸酵素を触媒に用いた乳酸と二酸化炭素からのリンゴ酸への電解合成
- Expression of MAGE-B Genes in Esophageal Squamous Cell Carcinoma
- Successive Hydrogenation of Styrene Using a Two-Compartment Cell Separated by Hydrogen Storage Alloy Sheet Electrodes
- Identification of de novo STAT3 target gene in liver regeneration
- Tooth pulp neurons in the caudal medulla oblongata of the cat
- Effect of ipriflavone in combination with calcium supplements on bone metabolism in elderly osteoporotic women
- A Measurement on Sensitivity Factors of Polyvinylidene Fluoride (PVDF) Transducers (Short Note)
- Distribution of Anionic Sites during Increasing Tight Junctional Permeability in the Rat Submandibular Gland
- Effect of Heating Value on Contact Diameter at Low Speed Breaking Contact
- The Relationship between Voltage and Duration of Short-Time Arc Generated by Slowly Breaking Silver Contact
- Matrix metalloproteinase 1 produced by CXCL12 stimulation on NK92 cells
- Improved Recovery of Human Urinary Protein for Electrophoresis
- Identification of Enolated 2-Thioxo-3-pyrrolidinecarbaldehyde, a New Degradation Product of 4-Methylthio-3-butenyl Isothiocyanate(Food & Nutrition)
- Formation of Yellow Pigment by the Reaction of 4-Methylthio-3-butenyl Isothiocyanate with L-Ascorbic Acid and Some Dihydroxyphenolic Compounds(Food & Nutrition)
- Total Synthesis and Chemical Biology of the Sarcodictyins
- Differential Inhibitory Mechanisms in VPL versus Intralaminar Nociceptive Neurons of the Cat : II. Effects of Systemic Morphine and CCK
- Efficacy of 5-fluorouracil on Tumor Growth in Walker -256 Xenografted rat. Mice is dependent on Circadian Rhythm
- Functional properties of nociceptive neurons responsive to dorsal neck muscle stimulation
- Inhibitory regulation by GABA_A receptors in the rat submandibular gland
- Intraductal injection of botulinum toxin induces functional changes in rat submandibular gland cells
- Effect of aquaporin 5 on paracellular water secretion in rat submandibular gland acini
- Effect of TGF-β on paracellular transport in the rat submandibular gland acini
- Inhibitory effects of vagal afferent inputs on nociceptive neurons responding to tooth pulp stimulation in the nucleus ventralis posteromedialis of the cat thalamus
- Regulated permeability of tight junctions by E-cadherin in rat submandibular gland acini
- Regulation by Cholecystokinin in Chorda-induced Salivary Secretion in the Rat Submandibular Gland
- Sex differences in odor-responding neurons of the rat medial amygdaloid area
- Effects of injection of botulinum toxin on nNOS in the rat submandibular gland
- Changes in salivary stress markers under experimental xerostomia in the rat
- Degranulation of Acinar Cells in von Ebner's Gland of the Rat
- Mechanisms of NK cell activation stimulated by CD2 : granzyme B is released by CD2 crosslinking-stimulation on NK92 cells
- Ablation of rat substantia nigra may provide a good acute model of Parkinson's disease for stem cell transplantation
- Mechanisms of NK cell activation stimulated by CD2 ; granzyme B is released by CD2 crosslinking-stimulation on NK92 cells