Mechanisms of NK cell activation stimulated by CD2 : granzyme B is released by CD2 crosslinking-stimulation on NK92 cells
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概要
- 論文の詳細を見る
Natural killer (NK) cells play a key role in the innate immune system through the rapid secretion of cytokines and the ability to lyse virally infected cells or tumor cells. CD2 are surface glycoprotein receptors and important for NK cell activation. Nevertheless, activation of NK92 cells through CD2 crosslinking-stimulation has not been completely understood. Here we analyzed the effects of CD2 crosslinking-stimulation on NK92 cell activation. We confirmed the expression of CD2 on the NK92 cell surface. The majority of NK92 cells expressed CD2 on their cell surfaces. We found that CD2 crosslinking-stimulation enhanced release of granzyme B from NK92 cells. CD2 crosslinking-stimulation markedly induced phosphorylation of extracellular signal-regulated kinase (ERK)1/2 compared with nonstimulated NK92 cells. Furthermore, we discovered that tyrosine phosphorylation of ERK1/2 was quickly induced within 1 min in an antibody concentration dependent manner. Mitogen-activated protein kinase extracellular signal-regulated kinase (MEK) inhibitor U0126 is a chemically synthesized organic compound that inhibits activation of ERK1/2. U0126 treatment inhibited the release of granzyme B, compared with CD2 crosslinking-stimulated NK92 cells, in a concentration-dependent manner. These results suggest that CD2 crosslinking-stimulation enhances the release of granzyme B and phosphorylation of ERK1/2, which are critical events during NK cell-mediated cytotoxicity.
- 2012-10-00
著者
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Nishikawa Yasuo
Department of Physiology, Osaka Dental University
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Uchihashi Kenji
Department Of Physiology Osaka Dental University
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OHSHIMA Takashi
Department of Agricultural Chemistry, Utsunomiya University
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Fujimoto Tetsuya
Department Of Chemistry Faculty Of Science Yamagata University
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Suwabe Takeshi
Department Of Physiology Osaka Dental University
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Inoue Hiroshi
Departmcnt Of Biology Faculty Of Science Kyushu University:(present)department Of Biology Kyushu Den
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Meng Shu
State Key Laboratory of Oral Diseases, Sichuan University:Department of Periodontology, West China College of Stomatology, Sichuan University
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Nishikawa Yasuo
Department of Electronic Engineering, Faculty of Engineering, Tokyo Institute of Polytechnics,
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Zhao Lei
State Key Laboratory of Oral Diseases, Sichuan University:Department of Periodontology, West China College of Stomatology, Sichuan University
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Wu Ya-fei
State Key Laboratory of Oral Diseases, Sichuan University:Department of Periodontology, West China College of Stomatology, Sichuan University
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Zhang Lin
State Key Laboratory of Oral Diseases, Sichuan University
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Inoue Hiroshi
Department of Physiology, Osaka Dental University
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Fang Yi-ru
Dental Oriental Medicine, Osaka Dental University
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Hirano Shun-ichiro
Department of Physiology, Osaka Dental University
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Aachi Tetsuya
Department of Physiology, Osaka Dental University
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Suwabe Takeshi
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
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Uchihashi Kenji
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
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Hirano Shun-ichiro
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
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ZHANG Lin
State Key Laboratory for Advanced Metals and Materials, Beijing Key Laboratory for Powder Metallurgy and Particulate Materials, University of Science and Technology Beijing
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Inoue Hiroshi
Deparment of Neurosurgery, Gunma University School of Medicine and Gamma Unit Center, Hidaka Hospital
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MENG Shu
State Key Laboratory of Oral Diseases, Sichuan University
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ZHAO Lei
State Key Laboratory of Oral Diseases, Sichuan University
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