Effect of TGF-β on paracellular transport in the rat submandibular gland acini

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We examined the influence of transforming growth factor (TGF)-β1 on the cells cell-cell interaction of submandibular gland cells, with particular emphasis on the regulation of junctional complex formation. Male Wistar rats weighing 250-280 g were used. Tight junctional permeability was detected cytochemically using microperoxidase as a tracer. The distribution of TGF-β1, TGF-β type II receptor and junctional proteins were observed immunocytochemically. The granular convoluted tubule (GCT) cells were intensely immunoreactive for TGF-β1. In contrast, both granule depletion and immunoreactive reduction were induced by isoprenaline stimulation. In unstimulated cells, TGF-β type II receptor immunoelectron staining was detected on both the apical and basolateral cellular membrane of the acinar cells. The cytochemical tracer test showed that isoprenaline stimulation increased tight junctional permeability in both acinar and duct cells. Treatment of the basolateral membrane with TGF-β1 led to enhancement of tight junctional permeability and breakdown of the junctional complex in both the tight and adherens junctions. However, breakdown in the tight junctional complex was not seen in the cells with isoproterenol stimulation of the gland. These results suggest that assembly of the intercellular junction in the rat submandibular gland acinar cells may be affected by TGF-β1 induced by paracrine action of GCT cells.
大阪歯科学会の論文