Regulated permeability of tight junctions by E-cadherin in rat submandibular gland acini
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概要
- 論文の詳細を見る
We examined the relationship between E-cadherin (E-CD), protein kinase C (PKC) and assembly of the tight junction (Tj) in rat submandibular gland acinar cells. The junctional complex of the acinar cells was double-labeled with anti-ZO-1 antibody and anti-E-CD antibody. When Ringer's solution was intraductally injected into the main duct, ZO-1 labels were highly concentrated at the Tj zone, and the adherens junction (Aj) was exclusively labeled by E-CD. In addition, the Tj was impermeable to microperoxidase. Neither intraductal injection of anti-E-CD antibody solution nor infusion of carbachol produced labels for ZO-1 at the Tj zone, although these proteins were occasionally intermixed at the Aj zone. In these cases, the Tj was permeable to microperoxidase. lntraductal injection of anti-E-CD antibody solution with PKC agonist, resulted in a reduction of E-CD labels in the Aj zone, while the ZO-1 was labeled exclusively in the Tj zone. In this case, Tj was impermeable to microperoxidase. These results suggest that E-CD plays a major role in mediating intercellular physical adhesion, and that PKC may be active in signaling the pathway activated by E-CD-mediated cell-cell adhesion.
- 大阪歯科学会の論文
著者
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Uchihashi Kenji
Department of Physiology, Osaka Dental University
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Yoshida Yo
Department of Physiology, Osaka Dental University
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Uchihashi K
Department Of Physiology Osaka Dental University
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Uchihashi Kenji
Department Of Physiology Osaka Dental University
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Yoshida Y
Department Of Physiology Osaka Dental University
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Yoshida Yo
Department Of Physiology Osaka Dental University
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Murakami Hirotaka
Department of Physiology, Osaka Dental University
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Murakami Hirotaka
Department Of Physiology Osaka Dental University
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Uchihashi Kenji
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
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