Degranulation of Acinar Cells in von Ebner's Gland of the Rat

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We investigated the effect of sympathetic agonists, parasympathetic muscarinic agonists and substance P on depletion of secretory granules in acinar cells of rat von Ebner's gland. Drugs were injected intraperitoneally at several different concentrations. Antagonists were given 15 minutes before injection of the agonist, and the extent of depletion of secretory granules in glandular acini was calculated using a computerized color image analyzer. The specific α_2-sympathetic agonist clonidine and the β_1-sympathetic agonist dobutamine produced a depletion of secretory granules. When combined with injections of the α_2-sympathetic antagonist yohimbine and the β_1-sympathetic antagonist acebutolol, depletion of secretory granules was blocked. The parasympathetic muscarinic agonist carbachol also produced a depletion of secretory granules. QNB blocked the depletion caused by carbachol, while atropine partially inhibited depletion. The specific M_1-muscarinic agonist McN-A-343 caused some depletion, although there was no significant differences between it and the control. Complete depletion of the secretory granules was achieved by carbachol stimulation superimposed on substance P stimulation. We concluded that the activation of the sympathetic α_2- and β_1-receptors, as well as the M_2 (M_^<2β>)-muscarinic and substance P receptors, results in degranulation of acinar cells in von Ebner's gland of the rat.
大阪歯科学会の論文