Inhibitory regulation by GABA_A receptors in the rat submandibular gland
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概要
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We investigated that localization of immunoreactivity and expression of mRNA for the γ-aminobutyric acid (GABA)_A receptor-γ 1 and GABA transporter-3 by immunohistochemistry and in situ hybridization in the rat submandibular gland. Further, we analyzed the role of GABA on salivary secretion. Both the GABA_A receptor and GABA transporter were expressed in the acinar, striated duct, collecting duct and submandibular ganglion cells. The expression of mRNA for the receptor and transporter was mainly localized in the submandibular ganglion. No salivary secretion was observed by GABA (10^-8-10^-33M) perfusion in the absence of electrical stimulation of chorda (20 Hz, 2-3 V, 5ms). GABA (10^-6-10^-3M)significantly inhibited chorda-evoked salivary secretion (the parasympathetic secretory response). This inhibition, which was induced by GABA, was antagonized by the GABA_A receptor antagonist, bicuculline (10^-3 and 10^-6M) and picrotoxin (10^-3 and 10^-5M). Inaddition, GABA inhibited the atropine-resistant parasympathetic secretory response. The GABA receptor antagonist picrotoxin (10^-3M)increased in the salivary flow with chorda stimulation at 1 Hz. On the other hand, salivary secretion evoked by electrical stimulation of the superior cervical ganglion (sympathetic secretory response) was not inhibited byGABA. Both functional and histochemical findings revealed that parasympathetic inhibitory regulation via GABA_A receptors is present in the rat submandibular gland.
- 大阪歯科学会の論文
著者
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OHNISHI Akio
Department of Neurology, University of Occupational and Environmental Health
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Uchihashi Kenji
Department of Physiology, Osaka Dental University
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Eto Kenji
Graduate School of Dentistry(Oral Diagnosis), Osaka Dental University
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Hojo Hirokazu
Department Of Oral Diagnosis Osaka Dental University
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Eto Kenji
Graduate School Of Dentistry(oral Diagnosis) Osaka Dental University
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Aragaki Toshiaki
Graduate School Of Dentistry(oral Diagnosis) Osaka Dental University
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Uchihashi K
Department Of Physiology Osaka Dental University
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Uchihashi Kenji
Department Of Physiology Osaka Dental University
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Nishikawa Yasuo
Department Of Physiology Osaka Dental University
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Nishikawa Yasuo
Department Of Physiology Osaka Dental Univeristy
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Ohnishi Akio
Department Of Oral Diagnosis Osaka Dental University
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Takai Noriyasu
Department of Physiology, Osaka Dental University
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Takai N
Department Of Physiology Osaka Dental University
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Takai Noriyasu
Department Of Physiology Osaka Dental University
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Ohnishi Akio
Department Of Neurology University Of Occupational And Environmental Health
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Nishikawa Yasuo
Osaka Dental Univ. Osaka Jpn
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Nishikawa Yasuo
Department of Electronic Engineering, Faculty of Engineering, Tokyo Institute of Polytechnics,
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Uchihashi Kenji
Department of Physiology and Graduate School of Dentistry, Osaka Dental University
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